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. 1995 Aug 4;269(5224):679-82.
doi: 10.1126/science.7624797.

Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2

M Hanks  1 , W Wurst, L Anson-Cartwright, A B Auerbach, A L Joyner
Affiliations

Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2

M Hanks et al. Science. .

Abstract

The related mouse Engrailed genes En-1 and En-2 are expressed from the one- and approximately five-somite stages, respectively, in a similar presumptive mid-hindbrain domain. However, mutations in En-1 and En-2 produce different phenotypes. En-1 mutant mice die at birth with a large mid-hindbrain deletion, whereas En-2 mutants are viable, with cerebellar defects. To determine whether these contrasting phenotypes reflect differences in temporal expression or biochemical activity of the En proteins, En-1 coding sequences were replaced with En-2 sequences by gene targeting. This rescued all En-1 mutant defects, demonstrating that the difference between En-1 and En-2 stems from their divergent expression patterns.

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