Differential induction and regulation of c-jun, junB, junD and c-fos by human papillomavirus type 11 E5a oncoprotein
- PMID: 7595372
- DOI: 10.1099/0022-1317-76-11-2653
Differential induction and regulation of c-jun, junB, junD and c-fos by human papillomavirus type 11 E5a oncoprotein
Abstract
The E5a gene of human papillomavirus type 11 (HPV-11) is a transforming oncogene. In this study, we investigated the mechanism of E5a induced transformation. Our results show that the expression of c-jun and junB, but not junD, was activated by HPV-11 E5a in NIH 3T3 cells and human epidermal keratinocytes. However, the expression of c-fos was activated by E5a in NIH 3T3 cells, but not in keratinocytes. We further investigated the mechanism of c-jun and junB induction by E5a. The amount of c-jun and junB RNAs correlated with the amount of E5a RNA in the heavy metal inducible system. E5a constitutively activated the expression of c-jun and junB at the initiation of transcription level. In addition, analyses of the effect of serum on c-jun expression in E5a transformed human epidermal keratinocytes show that EGF might have a stimulatory effect on c-jun gene expression in E5a expressing keratinocytes.
Similar articles
-
Coincidental expression of E5a and c-jun in human papillomavirus type 6/11-infected condylomata.J Gen Virol. 1996 Jun;77 ( Pt 6):1145-9. doi: 10.1099/0022-1317-77-6-1145. J Gen Virol. 1996. PMID: 8683199
-
Protein kinase C-and ras-dependent activation of c-jun gene by human papillomavirus type 11 E5a oncoprotein.Cancer Lett. 1995 Aug 16;95(1-2):201-5. doi: 10.1016/0304-3835(95)03894-3. Cancer Lett. 1995. PMID: 7656231
-
E5a gene of human papillomavirus type 11 is required for initiation but not for maintenance of transformation in NIH 3T3 cells.J Gen Virol. 1994 Aug;75 ( Pt 8):1953-60. doi: 10.1099/0022-1317-75-8-1953. J Gen Virol. 1994. PMID: 8046397
-
The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation.Biochim Biophys Acta. 1991 Dec 10;1072(2-3):129-57. doi: 10.1016/0304-419x(91)90011-9. Biochim Biophys Acta. 1991. PMID: 1751545 Review. No abstract available.
-
Molecular targets of the oncogenic transcription factor jun.Curr Cancer Drug Targets. 2003 Feb;3(1):41-55. doi: 10.2174/1568009033333781. Curr Cancer Drug Targets. 2003. PMID: 12570660 Review.
Cited by
-
Echovirus 1 replication, not only virus binding to its receptor, VLA-2, is required for the induction of cellular immediate-early genes.J Virol. 1997 May;71(5):4176-80. doi: 10.1128/JVI.71.5.4176-4180.1997. J Virol. 1997. PMID: 9094704 Free PMC article.
-
E5 proteins of human papillomavirus types 11 and 16 transactivate the c-fos promoter through the NF1 binding element.J Virol. 1996 Dec;70(12):8558-63. doi: 10.1128/JVI.70.12.8558-8563.1996. J Virol. 1996. PMID: 8970980 Free PMC article.
-
The E5 gene product of rhesus papillomavirus is an activator of endogenous Ras and phosphatidylinositol-3'-kinase in NIH 3T3 cells.Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12879-84. doi: 10.1073/pnas.93.23.12879. Proc Natl Acad Sci U S A. 1996. PMID: 8917513 Free PMC article.
-
Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts.Cancer Cell Int. 2006 Aug 9;6:19. doi: 10.1186/1475-2867-6-19. Cancer Cell Int. 2006. PMID: 16899131 Free PMC article.
-
Deciphering the divergent transcriptomic landscapes of cervical cancer cells grown in 3D and 2D cell culture systems.Front Cell Dev Biol. 2024 Aug 13;12:1413882. doi: 10.3389/fcell.2024.1413882. eCollection 2024. Front Cell Dev Biol. 2024. PMID: 39193365 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous