Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jul;14(7):618-21.
doi: 10.1007/BF01690738.

Detection of pathogenic fungi in human blood by the polymerase chain reaction

A M Polanco  1 , J L Rodríguez-Tudela, J V Martínez-Suárez
Affiliations

Detection of pathogenic fungi in human blood by the polymerase chain reaction

A M Polanco et al. Eur J Clin Microbiol Infect Dis. 1995 Jul.

Abstract

The ability of the polymerase chain reaction (PCR) to detect pathogenic fungi in human blood was investigated. A DNA fragment of about 300 bp from the 18S rDNA, highly conserved in all fungi, was amplified with target DNA from 18 different species of fungi commonly isolated from clinical samples. The presence of PCR products was confirmed by hybridization with a fluorescein-labelled internal probe (21-mer). The PCR assay described is sensitive enough to detect 125 fg of purified Candida albicans DNA and 10 to 100 yeast cells per millilitre of blood.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Clin Microbiol. 1991 Apr;29(4):810-2 - PubMed
    1. Nucleic Acids Res. 1991 Apr 25;19 Suppl:1987-2015 - PubMed
    1. J Med Vet Mycol. 1993;31(1):65-75 - PubMed
    1. Eur J Clin Microbiol Infect Dis. 1992 Apr;11(4):287-91 - PubMed
    1. J Med Microbiol. 1994 May;40(5):358-64 - PubMed

Publication types

LinkOut - more resources