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Comparative Study
. 1995 Sep 26;92(20):9097-101.
doi: 10.1073/pnas.92.20.9097.

Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3

T S Schaefer  1 , L K Sanders, D Nathans
Affiliations
Comparative Study

Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3

T S Schaefer et al. Proc Natl Acad Sci U S A. .

Abstract

To identify proteins that regulate the transcriptional activity of c-Jun, we have used the yeast two-hybrid screen to detect mammalian polypeptides that might interact functionally with the N-terminal segment of c-Jun, a known regulatory region. Among the proteins identified is a short form of Stat3 (designated Stat3 beta). Stat3 beta is missing the 55 C-terminal amino acid residues of the long form (Stat3 alpha) and has 7 additional amino acid residues at its C terminus. In the absence of added cytokines, expression of Stat3 beta (but not Stat3 alpha) in transfected cells activated a promoter containing the interleukin 6 responsive element of the rat alpha 2-macroglobulin gene; coexpression of Stat3 beta and c-Jun led to enhanced cooperative activation of the promoter. Nuclear extracts of cells transfected with a Stat3 beta expression plasmid formed a complex with an oligonucleotide containing a Stat3 binding site, whereas extracts of cells transfected with a Stat3 alpha plasmid did not. We conclude that there is a short form of Stat3 (Stat3 beta), that Stat3 beta is transcriptionally active under conditions where Stat3 alpha is not, and that Stat3 beta and c-Jun are capable of cooperative activation of certain promoters.

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References

    1. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1487-91 - PubMed
    1. Mol Cell Biol. 1988 Jan;8(1):466-72 - PubMed
    1. Nucleic Acids Res. 1989 Feb 11;17(3):1121-38 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1500-3 - PubMed
    1. Nature. 1989 Jul 20;340(6230):245-6 - PubMed

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