Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes
- PMID: 7566122
- DOI: 10.1038/377438a0
Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes
Abstract
In Xenopus oocytes, as well as other cells, inositol-1,4,5-trisphosphate (Ins(1,4,5)P3)-induced Ca2+ release is an excitable process that generates propagating Ca2+ waves that annihilate upon collision. The fundamental property responsible for excitability appears to be the Ca2+ dependency of the Ins(1,4,5)P3 receptor. Here we report that Ins(1,4,5)P3-induced Ca2+ wave activity is strengthened by oxidizable substrates that energize mitochondria, increasing Ca2+ wave amplitude, velocity and interwave period. The effects of pyruvate/malate are blocked by ruthenium red at the Ca2+ uniporter, by rotenone at complex I, and by antimycin A at complex III, and are subsequently rescued at complex IV by ascorbate tetramethylphenylenediamine (TMPD). Our data reveal that potential-driven mitochondrial Ca2+ uptake is a major factor in the regulation of Ins(1,4,5)P3-induced Ca2+ release and clearly demonstrate a physiological role of mitochondria in intracellular Ca2+ signalling.
Similar articles
-
Regulation of inositol trisphosphate-induced membrane currents in Xenopus oocytes by a Jurkat cell calcium influx factor.Biochem J. 1996 Sep 1;318 ( Pt 2)(Pt 2):649-56. doi: 10.1042/bj3180649. Biochem J. 1996. PMID: 8809059 Free PMC article.
-
Expression of inositol 1,4,5-trisphosphate receptors changes the Ca2+ signal of Xenopus oocytes.Am J Physiol. 1996 Apr;270(4 Pt 1):C1255-61. doi: 10.1152/ajpcell.1996.270.4.C1255. Am J Physiol. 1996. PMID: 8928753
-
Increased frequency of calcium waves in Xenopus laevis oocytes that express a calcium-ATPase.Science. 1993 Apr 9;260(5105):226-9. doi: 10.1126/science.8385800. Science. 1993. PMID: 8385800
-
Ca2+ wave dispersion and spiral wave entrainment in Xenopus laevis oocytes overexpressing Ca2+ ATPases.Biophys Chem. 1998 May 5;72(1-2):123-9. doi: 10.1016/s0301-4622(98)00128-8. Biophys Chem. 1998. PMID: 9652090 Review.
-
Spiral calcium waves: implications for signalling.Ciba Found Symp. 1995;188:66-77; discussion 78-84. doi: 10.1002/9780470514696.ch5. Ciba Found Symp. 1995. PMID: 7587624 Review.
Cited by
-
Molecular mechanism of mitochondrial calcium uptake.Cell Mol Life Sci. 2015 Apr;72(8):1489-98. doi: 10.1007/s00018-014-1810-1. Epub 2014 Dec 30. Cell Mol Life Sci. 2015. PMID: 25548802 Free PMC article. Review.
-
Supralinear Dependence of the IP3 Receptor-to-Mitochondria Local Ca2+ Transfer on the Endoplasmic Reticulum Ca2+ Loading.Contact (Thousand Oaks). 2024 Feb 14;7:25152564241229273. doi: 10.1177/25152564241229273. eCollection 2024 Jan-Dec. Contact (Thousand Oaks). 2024. PMID: 38362008 Free PMC article.
-
Dynamic visualization of calcium-dependent signaling in cellular microdomains.Cell Calcium. 2015 Oct;58(4):333-41. doi: 10.1016/j.ceca.2015.01.009. Epub 2015 Jan 29. Cell Calcium. 2015. PMID: 25703691 Free PMC article. Review.
-
Inhibitors of SERCA and mitochondrial Ca-uniporter decrease velocity of calcium waves in rat cardiomyocytes.Mol Cell Biochem. 2004 Jan-Feb;256-257(1-2):379-86. doi: 10.1023/b:mcbi.0000009883.71379.51. Mol Cell Biochem. 2004. PMID: 14977196
-
MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptake.Nature. 2010 Sep 16;467(7313):291-6. doi: 10.1038/nature09358. Epub 2010 Aug 8. Nature. 2010. PMID: 20693986 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous