Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Oct;15(10):5258-67.
doi: 10.1128/MCB.15.10.5258.

Regulation of interleukin 12 p40 expression through an NF-kappa B half-site

T L Murphy  1 , M G Cleveland, P Kulesza, J Magram, K M Murphy
Affiliations

Regulation of interleukin 12 p40 expression through an NF-kappa B half-site

T L Murphy et al. Mol Cell Biol. 1995 Oct.

Abstract

Interleukin 12 (IL-12) is an inducible cytokine composed of 35- and 40-kDa subunits that is critical for promoting T helper type 1 development and cell-mediated immunity against pathogens. The 40-kDa subunit, expressed by activated macrophages and B cells, is induced by several pathogens in vivo and in vitro and is augmented or inhibited by gamma interferon (IFN-gamma) or IL-10, respectively. Control of IL-12 p40 expression is therefore important for understanding resistance and susceptibility to a variety of pathogens, including Leishmania major and perhaps human immunodeficiency virus. In this report, we provide the first characterization of IL-12 p40 gene regulation in macrophages. We localize inducible activity of the promoter to the sequence -122GGGGAATTTTA-132 not previously recognized to bind Rel family transcription factors. We demonstrate binding of this sequence to NF-kappa B (p50/p65 and p50/c-Rel) complexes in macrophages activated by several p40-inducing pathogens and provide functional data to support a role for NF-kappa B family members in IL-12 p40 activation. Finally, we find that IFN-gamma treatment of cells enhances this binding interaction, thus potentially providing a mechanism for IFN-gamma augmentation of IL-12 production by macrophages.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Exp Med. 1991 Jul 1;174(1):73-81 - PubMed
    1. J Immunol. 1992 Jun 1;148(11):3433-40 - PubMed
    1. Science. 1992 Jun 5;256(5062):1452-6 - PubMed
    1. J Exp Med. 1992 Nov 1;176(5):1387-98 - PubMed
    1. Cell. 1992 Nov 27;71(5):777-89 - PubMed

Publication types

MeSH terms

Associated data