doi: 10.1002/eji.1830250302.
Antibodies that activate beta 2 integrins can generate different ligand binding states
Affiliations
- PMID: 7535698
- DOI: 10.1002/eji.1830250302
Item in Clipboard
Antibodies that activate beta 2 integrins can generate different ligand binding states
Eur J Immunol.
1995 Mar.
Abstract
A human erythroleukemic cell line (K562) that does not normally express beta 2 integrins has been transfected with the genes encoding these integrins. The resulting cell lines show minimal background adhesion but can be stimulated to bind to appropriate substrates when activated with either of two different antibodies to CD18. The two antibodies appear to generate different ligand binding states in LFA-1 such that different members of the ICAM family are recognized. Antibody-activated complement receptor type 3 and p150,95-transfected cells bind protein-coated surfaces, although they require slightly different activation conditions for optimal binding.
Similar articles
-
Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18.J Immunol. 1995 Jul 15;155(2):854-66. J Immunol. 1995. PMID: 7608563
-
T cell adhesion to intercellular adhesion molecule-1 (ICAM-1) is controlled by cell spreading and the activation of integrin LFA-1.J Immunol. 1996 Mar 1;156(5):1810-7. J Immunol. 1996. PMID: 8596031
-
Leucocyte/endothelial cell adhesion receptors in muscle biopsies from patients with idiopathic inflammatory myopathies (IIM).Clin Exp Immunol. 1996 Jun;104(3):467-73. Clin Exp Immunol. 1996. PMID: 9099932
-
Current status review: adhesion molecules and myelomonocytic cell-endothelial interactions.Br J Exp Pathol. 1989 Jun;70(3):385-94. Br J Exp Pathol. 1989. PMID: 2669925 Free PMC article. Review. No abstract available.
-
Detecting rapid and transient upregulation of leukocyte integrin affinity induced by chemokines and chemoattractants.J Immunol Methods. 2003 Feb;273(1-2):43-52. doi: 10.1016/s0022-1759(02)00417-9. J Immunol Methods. 2003. PMID: 12535796 Review.
Cited by
-
Advances in animal cell recombinant protein production: GS-NS0 expression system.Cytotechnology. 2000 Feb;32(2):109-23. doi: 10.1023/A:1008170710003. Cytotechnology. 2000. PMID: 19002973 Free PMC article.
-
Cytoplasmic tails of beta 1, beta 2, and beta 7 integrins differentially regulate LFA-1 function in K562 cells.Mol Biol Cell. 1997 Apr;8(4):719-28. doi: 10.1091/mbc.8.4.719. Mol Biol Cell. 1997. PMID: 9247650 Free PMC article.
-
Soluble beta-glucan polysaccharide binding to the lectin site of neutrophil or natural killer cell complement receptor type 3 (CD11b/CD18) generates a primed state of the receptor capable of mediating cytotoxicity of iC3b-opsonized target cells.J Clin Invest. 1996 Jul 1;98(1):50-61. doi: 10.1172/JCI118777. J Clin Invest. 1996. PMID: 8690804 Free PMC article.
-
A simple, no-wash cell adhesion-based high-throughput assay for the discovery of small-molecule regulators of the integrin CD11b/CD18.J Biomol Screen. 2007 Apr;12(3):406-17. doi: 10.1177/1087057106299162. J Biomol Screen. 2007. PMID: 17438069 Free PMC article.
-
T lymphocyte adhesion mechanisms within inflamed human kidney: studies with a Stamper-Woodruff assay.Am J Pathol. 1999 Feb;154(2):503-14. doi: 10.1016/S0002-9440(10)65296-0. Am J Pathol. 1999. PMID: 10027408 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
