Inhibition of endothelial-derived nitric oxide promotes P-selectin expression and actions in the rat microcirculation
- PMID: 7523213
- DOI: 10.1016/0016-5085(94)90229-1
Inhibition of endothelial-derived nitric oxide promotes P-selectin expression and actions in the rat microcirculation
Abstract
Background/aims: Inhibition of nitric oxide synthesis increases leukocyte and endothelial interaction in mesenteric venules. In this study, the relationship between inhibition of NO and expression of the adhesion molecule P-selectin was examined.
Methods: The rat mesentery was superfused with the NO inhibitor NG-nitro-L-arginine methyl ester (L-NAME) either alone or in combination with intravenous infusions of L-arginine, D-arginine, a P-selectin-neutralizing monoclonal antibody (PB1.3 [1 mg/kg]), recombinant human superoxide dismutase (hSOD), or 8 bromoguanosine 3',5'-cyclic monophosphate (8-br-cGMP). Leukocyte rolling and adherence were monitored in mesenteric venules via intravital microscopy. Ileal tissue superfused with L-NAME was examined immunohistochemically for P-selectin expression.
Results: Superfusion of the rat mesentery with L-NAME resulted in a significant increase in leukocyte rolling and adherence in the mesenteric venule, which was attenuated by administration of L-arginine but not D-arginine. Monoclonal antibody PB1.3 as well as hSOD and 8-br-cGMP administered before initiation of L-NAME superfusion significantly attenuated the increase in leukocyte rolling and adherence. Immunohistochemistry showed a significant increase in P-selectin expression after 60 minutes of superfusion with L-NAME, which was attenuated by L-arginine, hSOD, and 8-br-cGMP.
Conclusions: These data indicate an important functional relationship between endothelial-derived NO production and expression of the endothelial adhesion molecule P-selectin.
Comment in
-
Nitric oxide: regulator of P-selectin expression.Gastroenterology. 1994 Oct;107(4):1199-201. Gastroenterology. 1994. PMID: 7523220 No abstract available.
Similar articles
-
Inhibition of nitric oxide production. Mechanisms of vascular albumin leakage.Circ Res. 1993 Jul;73(1):164-71. doi: 10.1161/01.res.73.1.164. Circ Res. 1993. PMID: 7685251
-
Effects of defibrotide on leukocyte-endothelial cell interaction in the rat mesenteric vascular bed: role of P-selectin.Methods Find Exp Clin Pharmacol. 1996 Dec;18(10):669-76. Methods Find Exp Clin Pharmacol. 1996. PMID: 9121223
-
Leukocyte adhesion induced by inhibition of nitric oxide production in skeletal muscle.J Appl Physiol (1985). 1995 May;78(5):1725-32. doi: 10.1152/jappl.1995.78.5.1725. J Appl Physiol (1985). 1995. PMID: 7544340
-
Oligotide attenuates leukocyte-endothelial cell interaction via P-selectin in the rat mesenteric vascular bed.Eur J Pharmacol. 1996 Jan 25;296(2):181-7. doi: 10.1016/0014-2999(95)00695-8. Eur J Pharmacol. 1996. PMID: 8838455
-
Lysophosphatidylcholine stimulates leukocyte rolling and adherence in rat mesenteric microvasculature.Am J Physiol. 1997 Jun;272(6 Pt 2):H2584-90. doi: 10.1152/ajpheart.1997.272.6.H2584. Am J Physiol. 1997. PMID: 9227534
Cited by
-
Peroxynitrite inhibits leukocyte-endothelial cell interactions and protects against ischemia-reperfusion injury in rats.J Clin Invest. 1997 Feb 15;99(4):684-91. doi: 10.1172/JCI119212. J Clin Invest. 1997. PMID: 9045871 Free PMC article.
-
The effects of inflammatory cytokines on lymphatic endothelial barrier function.Angiogenesis. 2014 Apr;17(2):395-406. doi: 10.1007/s10456-013-9393-2. Epub 2013 Oct 20. Angiogenesis. 2014. PMID: 24141404 Free PMC article.
-
Deciphering and reversing tumor immune suppression.Immunity. 2013 Jul 25;39(1):61-73. doi: 10.1016/j.immuni.2013.07.005. Immunity. 2013. PMID: 23890064 Free PMC article. Review.
-
Nitric Oxide Donors in Endotoxic and Septic Shock: Evidence Against Nitric Oxide as a Mediator of Shock.Sepsis (Boston). 1998;1(2):101-106. doi: 10.1023/A:1009724424356. Sepsis (Boston). 1998. PMID: 32288618 Free PMC article. No abstract available.
-
Effect of some natural products either alone or in combination on gastritis induced in experimental rats.Dig Dis Sci. 2008 Jul;53(7):1774-84. doi: 10.1007/s10620-008-0246-6. Epub 2008 Mar 27. Dig Dis Sci. 2008. PMID: 18368490
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous