Nitric oxide as a messenger molecule for myoblast fusion
- PMID: 7514168
Nitric oxide as a messenger molecule for myoblast fusion
Abstract
Nitric oxide (NO) is a messenger molecule of vascular endothelial cells, macrophages, and neurons. Here, we demonstrate that the activity of NO synthase increases transiently but dramatically in chick embryonic myoblasts that are competent for fusion. This activity requires Ca2+, calmodulin, and NADPH. In addition, the increase in NO synthase activity coincides with an increase in cellular cGMP level. Furthermore, NO generated by treatment with sodium nitroprusside induces precocious myoblast fusion, while treatment with NG-monomethyl-L-arginine, a competitive inhibitor of NO synthase, or methylene blue, an inhibitor of guanylate cyclase, delays fusion. These results provide the first evidence for a strong association of NO with myoblast fusion.
Similar articles
-
Muscarinic cholinergic stimulation of the nitric oxide-cyclic GMP signaling system in cultured rat sensory neurons.Neuroscience. 1994 Sep;62(2):351-9. doi: 10.1016/0306-4522(94)90370-0. Neuroscience. 1994. PMID: 7530344
-
Nitric oxide as a signal in thyroid.Mol Cell Endocrinol. 1992 Dec;90(1):R1-3. doi: 10.1016/0303-7207(92)90115-m. Mol Cell Endocrinol. 1992. PMID: 1284493
-
Involvement of cyclic GMP in the fusion of chick embryonic myoblasts in culture.Exp Cell Res. 1992 Mar;199(1):129-33. doi: 10.1016/0014-4827(92)90470-s. Exp Cell Res. 1992. PMID: 1310470
-
Possible role of nitric oxide in catecholamine secretion by chromaffin cells in the presence and absence of cultured endothelial cells.J Neurochem. 1994 Sep;63(3):988-96. doi: 10.1046/j.1471-4159.1994.63030988.x. J Neurochem. 1994. PMID: 7519669
-
Cell death, survival and proliferation in Tetrahymena thermophila. Effects of insulin, sodium nitroprusside, 8-Bromo cyclic GMP, NG-methyl-L-arginine and methylene blue.Cell Biol Int. 1996 Oct;20(10):653-66. doi: 10.1006/cbir.1996.0087. Cell Biol Int. 1996. PMID: 8969458 Review.
Cited by
-
Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production.J Muscle Res Cell Motil. 2006;27(8):577-84. doi: 10.1007/s10974-006-9078-1. Epub 2006 Oct 19. J Muscle Res Cell Motil. 2006. PMID: 17051348
-
NF-kappaB functions in stromal fibroblasts to regulate early postnatal muscle development.J Biol Chem. 2010 Feb 19;285(8):5479-87. doi: 10.1074/jbc.M109.075606. Epub 2009 Dec 14. J Biol Chem. 2010. PMID: 20018862 Free PMC article.
-
Nitric oxide stimulates glucose transport and metabolism in rat skeletal muscle in vitro.Biochem J. 1997 Feb 15;322 ( Pt 1)(Pt 1):223-8. doi: 10.1042/bj3220223. Biochem J. 1997. PMID: 9078265 Free PMC article.
-
Study of muscle contraction induced by electrical pulse stimulation and nitric oxide in C2C12 myotube cells.J Exerc Nutrition Biochem. 2018 Mar 30;22(1):22-28. doi: 10.20463/jenb.2018.0004. J Exerc Nutrition Biochem. 2018. PMID: 29673242 Free PMC article.
-
Targeting angiogenesis in Duchenne muscular dystrophy.Cell Mol Life Sci. 2019 Apr;76(8):1507-1528. doi: 10.1007/s00018-019-03006-7. Epub 2019 Feb 15. Cell Mol Life Sci. 2019. PMID: 30770952 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
