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. 1995 Dec 1;182(6):1801-6.
doi: 10.1084/jem.182.6.1801.

Oncostatin M induces association of Grb2 with Janus kinase JAK2 in multiple myeloma cells

D Chauhan  1 , S M Kharbanda, A Ogata, M Urashima, D Frank, N Malik, D W Kufe, K C Anderson
Affiliations

Oncostatin M induces association of Grb2 with Janus kinase JAK2 in multiple myeloma cells

D Chauhan et al. J Exp Med. .

Abstract

Oncostatin M (OSM) is a 28-kD glycoprotein recently identified as a growth factor for human multiple myeloma cells. It belongs to a family of distantly related cytokines that includes interleukin 6, ciliary neurotrophic factor, leukemia-inhibitory factor, and interleukin 11. These cytokines initiate signaling by inducing either homodimerization of gp130 or heterodimerization of gp130 with leukemia-inhibitory factor receptor beta components. Such dimerization in turn activates receptor-associated tyrosine kinases. In the present study using U266B1 human multiple myeloma cells, we show that OSM induces tyrosine phosphorylation and activation of JAK2, but not JAK1 or Tyk2, kinases. The results also demonstrate that OSM induces direct interaction of JAK2 kinase with Grb2, an SH2/SH3 domain containing adaptor protein. The SH2 domain of Grb2 is directly associated with tyrosine-phosphorylated JAK2. Furthermore, the presence of Sos in the JAK2-Grb2 complex suggests a role for Ras in OSM-transduced signaling.

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References

    1. Mol Cell Biol. 1991 Sep;11(9):4371-9 - PubMed
    1. Cell. 1993 Jul 30;74(2):227-36 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11349-53 - PubMed
    1. Science. 1992 Mar 13;255(5050):1434-7 - PubMed
    1. Nature. 1992 Mar 26;356(6367):340-4 - PubMed

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