Non-oxidative loss of glutathione in apoptosis via GSH extrusion
- PMID: 7488106
- DOI: 10.1006/bbrc.1995.2626
Non-oxidative loss of glutathione in apoptosis via GSH extrusion
Abstract
Reduced glutathione (GSH) has been hypothesized to play a role in the rescue of cells from apoptosis, by buffering an endogenously induced oxidative stress. We correlated GSH levels and apoptosis in U937 human monocytic cells induced to apoptosis by different agents. All treatments led to depletion of GSH concomitant with the onset of apoptosis. The loss was due to extrusion of GSH outside the cell, while GSSG was not accumulated in the apoptosing cells, nor was it found in the extracellular medium. Modulation of intracellular GSH level did not influence the overall extent of apoptosis. We conclude that glutathione loss in apoptosis is not necessarily preceded by an oxidative stress, and that GSH depletion alone is not sufficient to lead cells to apoptosis.
Similar articles
-
Glutathione redox cycle-driven recovery of reduced glutathione after oxidation by tertiary-butyl hydroperoxide in preimplantation mouse embryos.Arch Biochem Biophys. 1995 Aug 1;321(1):6-12. doi: 10.1006/abbi.1995.1361. Arch Biochem Biophys. 1995. PMID: 7639536
-
Regulation of apoptosis/necrosis execution in cadmium-treated human promonocytic cells under different forms of oxidative stress.Apoptosis. 2006 May;11(5):673-86. doi: 10.1007/s10495-006-5879-3. Apoptosis. 2006. PMID: 16532269
-
Docosahexaenoic acid induces apoptosis in the human PaCa-44 pancreatic cancer cell line by active reduced glutathione extrusion and lipid peroxidation.Nutr Cancer. 2005;52(2):225-33. doi: 10.1207/s15327914nc5202_12. Nutr Cancer. 2005. PMID: 16201853
-
Glutathione modulation in cancer treatment: will it work?Int J Radiat Oncol Biol Phys. 1989 May;16(5):1289-95. doi: 10.1016/0360-3016(89)90301-5. Int J Radiat Oncol Biol Phys. 1989. PMID: 2654105 Review.
-
Glutathione metabolism.Methods Enzymol. 1995;251:3-7. doi: 10.1016/0076-6879(95)51106-7. Methods Enzymol. 1995. PMID: 7651209 Review. No abstract available.
Cited by
-
Cystathionine protects against endoplasmic reticulum stress-induced lipid accumulation, tissue injury, and apoptotic cell death.J Biol Chem. 2012 Sep 14;287(38):31994-2005. doi: 10.1074/jbc.M112.355172. Epub 2012 Aug 1. J Biol Chem. 2012. PMID: 22854956 Free PMC article.
-
Dose-Dependent Mutation Rates Determine Optimum Erlotinib Dosing Strategies for EGFR Mutant Non-Small Cell Lung Cancer Patients.PLoS One. 2015 Nov 4;10(11):e0141665. doi: 10.1371/journal.pone.0141665. eCollection 2015. PLoS One. 2015. PMID: 26536620 Free PMC article.
-
Oxidative stress-induced changes in pyridine nucleotides and chemoattractant 5-lipoxygenase products in aging neutrophils.Free Radic Biol Med. 2009 Jul 1;47(1):62-71. doi: 10.1016/j.freeradbiomed.2009.04.016. Epub 2009 Apr 17. Free Radic Biol Med. 2009. PMID: 19376220 Free PMC article.
-
Nanoceria protects from alterations in oxidative metabolism and calcium overloads induced by TNFα and cycloheximide in U937 cells: pharmacological potential of nanoparticles.Mol Cell Biochem. 2014 Dec;397(1-2):245-53. doi: 10.1007/s11010-014-2192-2. Epub 2014 Aug 23. Mol Cell Biochem. 2014. PMID: 25148872
-
Glutathione depletion in survival and apoptotic pathways.Front Pharmacol. 2014 Dec 4;5:267. doi: 10.3389/fphar.2014.00267. eCollection 2014. Front Pharmacol. 2014. PMID: 25538619 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
