Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1995 Nov;69(11):7152-8.
doi: 10.1128/JVI.69.11.7152-7158.1995.

Protective efficacy of nonneutralizing monoclonal antibodies in acute infection with murine leukemia virus

S H Pincus  1 , R Cole, R Ireland, F McAtee, R Fujisawa, J Portis
Affiliations
Comparative Study

Protective efficacy of nonneutralizing monoclonal antibodies in acute infection with murine leukemia virus

S H Pincus et al. J Virol. 1995 Nov.

Abstract

We have used an experimental retrovirus infection to study the roles played by different antibodies in resistance to both infection and disease. A molecularly cloned chimeric murine leukemia virus was used to induce acute lethal neurological disease in neonatal mice. A panel of monoclonal antibodies directed against the Gag and Env proteins was tested for protective efficacy. In vitro neutralization assays demonstrated that anti-Env antibodies gave different degrees of neutralization, while no anti-Gag neutralized the virus. In vivo experimental endpoints were onset of clinical signs and premoribund condition. As expected, different anti-Env antibodies demonstrated different degrees of protection which correlated with their neutralizing abilities. Surprisingly, anti-Gag antibodies directed against both p15 (MA protein) and p30 (CA protein) were also protective, significantly delaying the onset of disease. No protection was seen with either of two control antibodies. The protection with anti-Gag was dose related and time dependent and was also produced with Fab fragments. Treatment with anti-Gag did not prevent viremia but resulted in a slight slowing in viremia kinetics and decreased levels of virus in the central nervous systems of mice protected from disease. These data indicate that nonneutralizing antiretroviral antibodies can influence the outcome of retroviral disease. The data also suggest a functional role for cell surface expression of Gag proteins on murine leukemia virus-infected cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1979 Apr;30(1):255-66 - PubMed
    1. Cell. 1976 Dec;9(4 Pt 1):573-8 - PubMed
    1. Virology. 1981 Jul 15;112(1):131-44 - PubMed
    1. Nature. 1982 May 6;297(5861):70-2 - PubMed
    1. J Immunol. 1983 May;130(5):2363-7 - PubMed

Publication types

MeSH terms