Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Dec;115(2):310-21.
doi: 10.1016/0042-6822(81)90113-6.

RNA and polypeptide homology among murine coronaviruses

S Cheley, V L Morris, M J Cupples, R Anderson

RNA and polypeptide homology among murine coronaviruses

S Cheley et al. Virology. 1981 Dec.

Abstract

Using a 32P complementary DNA (cDNA) prepared against the A59 nucleocapsid protein messenger RNA, we have investigated the extent of homology between A59 and four other strains of murine hepatitis virus (MHV). Analysis by hybridization kinetics of the annealing between A59 [32P]cDNA and infected cell RNA from the other four MHV strains demonstrated 70–80% homology. By gel transfer analysis, the A59 [32P]cDNA was able to detect subgenomic-size virus-specific RNAs in cells infected with all of the five MHV strains. A similar pattern of seven viral RNAs was detected in cells infected with A59, MHV-1, MHV-3, and JHM. In contrast, cells infected with MHV-S contained seven virus-specific RNAs, of which only the two smallest species comigrated with RNAs from the other four strains. The results suggest that as previously shown with A59 (S. Cheley, R. Anderson, M. J. Cupples, E. C. M. Lee Chan, and V. L. Morris (1981)Virology, 112, 596–604), all MHV strains tested encode a nested set of subgenomic RNAs. Analysis of [35S]methionine-labeled viral proteins by SDS-polyacrylamide gel electrophoresis revealed that each strain of MHV specified four major viral polypeptides with apparent molecular weights very similar to those previously reported for the E2, N, El, and PEI polypeptides of A59. The strong degree of interstrain homology among the five MHV strains investigated was confirmed by comparative chymotryptic peptide mapping of the viral N proteins. A majority of the chymotryptic peptides from each of the [35Sknethionine-labeled N proteins was found to coelute by high-performance liquid chromotography. Moreover, this technique of peptide mapping indicated a particularly strong relatedness between MHV-1 and MHV-S and among MHV-3, JHM, and A59.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Albino A., Korngold L., Mellors R.C. Tryptic peptide analysis of gag gene proteins of endogenous mouse type C viruses. J. ViroL. 1979;29:102–113. - PMC - PubMed
    1. Anderson R., Cheley S., Haworth-Hatherell E. Comparison of polypeptides of two strains of murine hepatitis virus. Virology. 1979;97:492–494. - PubMed
    1. Bailey O.T., Pappenheimer A.M., Cheever F.S., Daniels J.B. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. J. Exp. Med. 1949;90:195–212. - PMC - PubMed
    1. Bang F.B., Warwick A. Vol. 46. 1960. Mouse macrophages as host for the mouse hepatitis virus and the genetic basis of their susceptibility; pp. 1065–1075. (Proc Nat. Acad. Sci USA). - PMC - PubMed
    1. Barbacid M., Stephenson J.R., Aaronson S.A. Evolutionary relationships between gag gene coded proteins of murine and primate endogenous type C RNA viruses. Cell. 1977;10:641–648. - PubMed

Publication types

LinkOut - more resources