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. 1981 Jun;24(3):729-39.
doi: 10.1016/0092-8674(81)90099-4.

M-MuLV-induced leukemogenesis: integration and structure of recombinant proviruses in tumors

H van der Putten, W Quint, J van Raaij, E R Maandag, I M Verma, A Berns

M-MuLV-induced leukemogenesis: integration and structure of recombinant proviruses in tumors

H van der Putten et al. Cell. 1981 Jun.

Abstract

M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral sequences. All reintegrated genomes carry long terminal repeat (LTR) sequences at both termini of their genome. In the preleukemic stage a large population of cells exhibiting a random distribution of reintegrated M-MuLV genomes are seen, but during outgrowth of the tumor, selection of cells occurs leaving one or a few clonal descendants in the outgrown tumor. In this latter stage recombinant genomes can be detected. Although these recombinants constitute a heterogeneous group of proviruses, characteristic molecular markers are conserved among many individual proviral recombinants, lending credence to the notion that a certain recombinant structure is a prerequisite for the onset of neoplasia. The structure of these recombinants shows close structural similarities to the previously described mink cell focus-inducing (MCF)-type viruses.

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