Organization of rhodopsin and a high molecular weight glycoprotein in rod photoreceptor disc membranes using monoclonal antibodies
- PMID: 7085619
Organization of rhodopsin and a high molecular weight glycoprotein in rod photoreceptor disc membranes using monoclonal antibodies
Abstract
Four monoclonal antibodies obtained from the fusion of mouse myeloma cells with lymphocytes of mice immunized with bovine rod outer segment disc membranes were shown to bind to the surface of sealed discs. Radioimmune labeling of rod outer segment membrane proteins separated by sodium dodecyl sulfate gel electrophoresis indicated that two monoclonal antibodies (3D6 and 4B4) were against rhodopsin. Limited proteolysis of rod outer segment membranes with trypsin and Streptomyces griseus protease indicated that the 3D6 antibody bound to the trypsin-sensitive region close to the carboxyl-terminal end of rhodopsin. The 4B4 antibody bound at a trypsin insensitive, but S. griseus protease-sensitive internal region of rhodopsin accessible on the cytoplasmic surface of discs. Two other monoclonal antibodies (3D12 and 4B2) were found to bind to different regions of the Mr = 220,000 concanavalin A binding glycoprotein of rod outer segment disc membranes. Proteolysis studies indicated that these antibodies also bound to a Mr = 140,000 fragment which does not contain the concanavalin A binding site. Immunoferritin-labeling studies for transmission electron microscopy confirm the location of the 3D6 and 4B2 antigens on the cytoplasmic or interdisc surface of disc membranes.
Similar articles
-
Orientation of membrane glycoproteins in sealed rod outer segment disks.Biochemistry. 1979 Dec 25;18(26):5868-73. doi: 10.1021/bi00593a017. Biochemistry. 1979. PMID: 518872 No abstract available.
-
Differential immunogold-dextran labeling of bovine and frog rod and cone cells using monoclonal antibodies against bovine rhodopsin.Exp Eye Res. 1986 Jan;42(1):55-71. doi: 10.1016/0014-4835(86)90017-5. Exp Eye Res. 1986. PMID: 2420630
-
Peripherin. A rim-specific membrane protein of rod outer segment discs.Invest Ophthalmol Vis Sci. 1987 Jan;28(1):50-61. Invest Ophthalmol Vis Sci. 1987. PMID: 2433249
-
Differences in the protein composition of bovine retinal rod outer segment disk and plasma membranes isolated by a ricin-gold-dextran density perturbation method.J Cell Biol. 1987 Dec;105(6 Pt 1):2589-601. doi: 10.1083/jcb.105.6.2589. J Cell Biol. 1987. PMID: 2447095 Free PMC article.
-
Understanding the Rhodopsin Worldview Through Atomic Force Microscopy (AFM): Structure, Stability, and Activity Studies.Chem Rec. 2023 Oct;23(10):e202300113. doi: 10.1002/tcr.202300113. Epub 2023 Jun 2. Chem Rec. 2023. PMID: 37265335 Free PMC article. Review.
Cited by
-
RD3, the protein associated with Leber congenital amaurosis type 12, is required for guanylate cyclase trafficking in photoreceptor cells.Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21158-63. doi: 10.1073/pnas.1010460107. Epub 2010 Nov 15. Proc Natl Acad Sci U S A. 2010. PMID: 21078983 Free PMC article.
-
Secretory Carrier Membrane Protein 2 Regulates Cell-surface Targeting of Brain-enriched Na+/H+ Exchanger NHE5.J Biol Chem. 2009 May 15;284(20):13892-13903. doi: 10.1074/jbc.M807055200. Epub 2009 Mar 10. J Biol Chem. 2009. PMID: 19276089 Free PMC article.
-
Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.J Biol Chem. 2011 May 13;286(19):17205-16. doi: 10.1074/jbc.M111.229419. Epub 2011 Mar 18. J Biol Chem. 2011. PMID: 21454556 Free PMC article.
-
Anti-lamprey retinal antibodies: immunohistochemistry on the retinas of several species of vertebrates.Cell Tissue Res. 1991 Dec;266(3):419-26. doi: 10.1007/BF00318583. Cell Tissue Res. 1991. PMID: 1811877
-
Distribution of the Na(+)-Ca2+ exchange protein in mammalian cardiac myocytes: an immunofluorescence and immunocolloidal gold-labeling study.J Cell Biol. 1992 Apr;117(2):337-45. doi: 10.1083/jcb.117.2.337. J Cell Biol. 1992. PMID: 1373142 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
