Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis
- PMID: 7017165
- PMCID: PMC171239
- DOI: 10.1128/JVI.38.3.996-1004.1981
Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis
Abstract
A 65,000-dalton (65K) antigen found in Raji cells by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis has been identified as an Epstein-Barr virus nuclear antigen (EBNA). This identification is based on the following evidence. The 65K antigen is detected in Raji cells but not in three Epstein-Barr virus (-) human B cell lines. It is not detected with EBNA (-) sera. The 65K antigen is found predominantly in the nucleus and co-elutes with EBNA during partial purification by DNA-Sepharose and Blue Dextran-Sepharose chromatography. Finally, the partially purified 65K antigen is an effective absorbant of EBNA antibody as measured in an anticomplement immunofluorescence assay. Antigens with molecular weights of 72, 70, and 73K have been detected in B95-8, P3HR-1, and Namalwa cells, respectively. These antigens are the likely homologues of the 65K Raji EBNA. In addition, an Epstein-Barr virus-associated, 81K DNA-binding antigen has been detected in both B95-8 and Raji cells.
Similar articles
-
Partial purification of the Epstein-Barr virus nuclear antigen(s).J Biol Chem. 1983 Mar 25;258(6):3974-82. J Biol Chem. 1983. PMID: 6300065
-
Purification of the Epstein-Barr virus-determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines.J Virol. 1978 Sep;27(3):604-11. doi: 10.1128/JVI.27.3.604-611.1978. J Virol. 1978. PMID: 212583 Free PMC article.
-
Characterization of Epstein-Barr virus nuclear antigen(s) in different cell lines by radioimmunoelectrophoresis.Intervirology. 1984;22(4):191-200. doi: 10.1159/000149551. Intervirology. 1984. PMID: 6096291
-
The Epstein-Barr virus-determined nuclear antigen: a previously identified 48K component and higher-molecular-weight forms of the antigen are structurally related.Intervirology. 1983;20(4):213-22. doi: 10.1159/000149394. Intervirology. 1983. PMID: 6317606
-
Monoclonal and polyclonal antibodies against Epstein-Barr virus nuclear antigen 5 (EBNA-5) detect multiple protein species in Burkitt's lymphoma and lymphoblastoid cell lines.J Virol. 1987 Dec;61(12):3870-8. doi: 10.1128/JVI.61.12.3870-3878.1987. J Virol. 1987. PMID: 2824821 Free PMC article.
Cited by
-
Constitutive expression of Epstein-Barr virus-encoded RNAs and nuclear antigen during latency and after induction of Epstein-Barr virus replication.J Virol. 1985 Jan;53(1):254-9. doi: 10.1128/JVI.53.1.254-259.1985. J Virol. 1985. PMID: 2981344 Free PMC article.
-
Identification and expression of a nuclear antigen from the genomic region of the Jijoye strain of Epstein-Barr virus that is missing in its nonimmortalizing deletion mutant, P3HR-1.Proc Natl Acad Sci U S A. 1985 Nov;82(21):7429-33. doi: 10.1073/pnas.82.21.7429. Proc Natl Acad Sci U S A. 1985. PMID: 2997790 Free PMC article.
-
Expression of a second Epstein-Barr virus-determined nuclear antigen in mouse cells after gene transfer with a cloned fragment of the viral genome.Proc Natl Acad Sci U S A. 1985 May;82(10):3435-9. doi: 10.1073/pnas.82.10.3435. Proc Natl Acad Sci U S A. 1985. PMID: 2987926 Free PMC article.
-
Identification of Epstein-Barr nuclear antigen polypeptide in mouse and monkey cells after gene transfer with a cloned 2.9-kilobase-pair subfragment of the genome.Proc Natl Acad Sci U S A. 1984 Jan;81(1):43-7. doi: 10.1073/pnas.81.1.43. Proc Natl Acad Sci U S A. 1984. PMID: 6320171 Free PMC article.
-
Antibody responses to Epstein-Barr virus-determined nuclear antigen (EBNA)-1 and EBNA-2 in acute and chronic Epstein-Barr virus infection.Proc Natl Acad Sci U S A. 1987 Jan;84(2):570-4. doi: 10.1073/pnas.84.2.570. Proc Natl Acad Sci U S A. 1987. PMID: 3025881 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources