Primary structure and evolution of rat growth hormone gene
- PMID: 6946433
- PMCID: PMC320277
- DOI: 10.1073/pnas.78.8.4867
Primary structure and evolution of rat growth hormone gene
Abstract
The rat growth hormone gene was isolated on a cloned 11.4-kilobase EcoRI-generated DNA fragment from a bacteriophage "library" of chromosomal DNA. The structural gene sequence, approximately 2.1 kilobases long, was identified by hybridization to the corresponding cloned rat growth hormone cDNA and shown to contain four intervening sequences. The complete primary structure of the gene and the 5' and 3'-flanking regions was determined. The mosaic structure of exons and introns can be related to the different biological activities of growth hormone and to the evolution from ancestral sequences of a gene that was the precursor to the growth hormone and the related prolactin and placental lactogen (chorionic somatomammotropin) genes. The largest intron was found to contain a dispersed repetitive DNA sequence flanked by perfect 18-base pair direct repeats. The mobility of sequences of this kind could play a role in the observed variation of intron sizes and in rearrangements of mammalian genes.
Similar articles
-
Structure of genes for human growth hormone and chorionic somatomammotropin.Proc Natl Acad Sci U S A. 1979 Sep;76(9):4294-8. doi: 10.1073/pnas.76.9.4294. Proc Natl Acad Sci U S A. 1979. PMID: 291965 Free PMC article.
-
Structure of a fish (rainbow trout) growth hormone gene and its evolutionary implications.Proc Natl Acad Sci U S A. 1988 Jul;85(14):5136-40. doi: 10.1073/pnas.85.14.5136. Proc Natl Acad Sci U S A. 1988. PMID: 3393535 Free PMC article.
-
Genomic organization of rat prolactin and growth hormone genes.Proc Natl Acad Sci U S A. 1980 Aug;77(8):4583-7. doi: 10.1073/pnas.77.8.4583. Proc Natl Acad Sci U S A. 1980. PMID: 6254031 Free PMC article.
-
Sequence-function relationships within the expanding family of prolactin, growth hormone, placental lactogen, and related proteins in mammals.Endocr Rev. 1996 Aug;17(4):385-410. doi: 10.1210/edrv-17-4-385. Endocr Rev. 1996. PMID: 8854051 Review. No abstract available.
-
Hormonal and cell-specific regulation of the human growth hormone and chorionic somatomammotropin genes.Prog Nucleic Acid Res Mol Biol. 1996;54:127-63. doi: 10.1016/s0079-6603(08)60362-1. Prog Nucleic Acid Res Mol Biol. 1996. PMID: 8768074 Review. No abstract available.
Cited by
-
Variability within the rabbit C repeats and sequences shared with other SINES.Nucleic Acids Res. 1985 Feb 25;13(4):1073-88. doi: 10.1093/nar/13.4.1073. Nucleic Acids Res. 1985. PMID: 4000935 Free PMC article.
-
Activin inhibits binding of transcription factor Pit-1 to the growth hormone promoter.Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11451-5. doi: 10.1073/pnas.89.23.11451. Proc Natl Acad Sci U S A. 1992. PMID: 1454833 Free PMC article.
-
Cell-specific expression of transfected brain identifier repetitive DNAs.Nucleic Acids Res. 1988 May 11;16(9):3963-76. doi: 10.1093/nar/16.9.3963. Nucleic Acids Res. 1988. PMID: 2453842 Free PMC article.
-
Repetitive DNA sequences within and around the rat prolactin gene.Mol Cell Biochem. 1985 Jan;65(2):171-9. doi: 10.1007/BF00221100. Mol Cell Biochem. 1985. PMID: 3982398
-
Stable accumulation of a rat truncated repeat transcript in Xenopus oocytes.Proc Natl Acad Sci U S A. 1986 May;83(10):3106-10. doi: 10.1073/pnas.83.10.3106. Proc Natl Acad Sci U S A. 1986. PMID: 2422646 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
