doi: 10.1093/nar/9.10.2387.
Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit
- PMID: 6910637
- PMCID: PMC326852
- DOI: 10.1093/nar/9.10.2387
Item in Clipboard
Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit
Nucleic Acids Res.
.
Free PMC article
Abstract
In the quaternary initiation complex, eIF-2.GMPPCP.Met-tRNAf.40S ribosomal subunit, the Met-tRNAf can be cross-linked to the beta subunit of initiation factor eIF-2 as well as to ribosomal proteins S3a and S6 by treatment with the bifunctional reagent, diepoxybutane. Using 40S subunits, modified in advance with the heterobifunctional reagent, methyl-rho-azido-benzoylaminoacetimidate, Met-tRNAf is covalently bound to the same ribosomal proteins (S3a and S6) upon irradiation of the complex with ultraviolet light. Under both conditions proteins S3a and S6, together with a limited number of other ribosomal proteins, are covalently bound to 18S ribosomal RNA.
Similar articles
-
Identification of proteins of the 40 S ribosomal subunit involved in interaction with initiation factor eIF-2 in the quaternary initiation complex by means of monospecific antibodies.FEBS Lett. 1988 Jun 6;233(1):114-8. doi: 10.1016/0014-5793(88)81366-8. FEBS Lett. 1988. PMID: 3384085
-
Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3 small ribosomal subunit, as shown by cross-linking experiments.Nucleic Acids Res. 1982 Feb 25;10(4):1327-34. doi: 10.1093/nar/10.4.1327. Nucleic Acids Res. 1982. PMID: 7071015 Free PMC article.
-
Phosphorylation of ribosomal proteins as a possible control system for protein synthesis. Binding of Met-tRNAf to 40 S ribosomal subunits.Acta Biol Med Ger. 1981;40(9):1105-10. Acta Biol Med Ger. 1981. PMID: 6918182
-
Eukaryotic initiation factors eIF-2 and eIF-3: interactions, structure and localization in ribosomal initiation complexes.Biochimie. 1991 Jul-Aug;73(7-8):1007-19. doi: 10.1016/0300-9084(91)90142-n. Biochimie. 1991. PMID: 1742346 Review.
-
The role of phosphorylation in the regulation of eukaryotic initiation factor 2 activity.Curr Top Cell Regul. 1982;21:35-63. doi: 10.1016/b978-0-12-152821-8.50006-2. Curr Top Cell Regul. 1982. PMID: 6291856 Review. No abstract available.
Cited by
-
Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein.Curr Genet. 1993;23(4):330-3. doi: 10.1007/BF00310894. Curr Genet. 1993. PMID: 8467530
-
Influence of the state of ribosome association on the phosphorylation of ribosomal proteins in isolated ribosome--protein kinase systems from rat cerebral cortex.Biochem J. 1982 Nov 15;208(2):289-300. doi: 10.1042/bj2080289. Biochem J. 1982. PMID: 6297465 Free PMC article.
-
Probing the structure of mouse Ehrlich ascites cell 5.8S, 18S and 28S ribosomal RNA in situ.Nucleic Acids Res. 1994 Apr 25;22(8):1374-82. doi: 10.1093/nar/22.8.1374. Nucleic Acids Res. 1994. PMID: 8190627 Free PMC article.
-
Altered cellular responses by varying expression of a ribosomal protein gene: sequential coordination of enhancement and suppression of ribosomal protein S3a gene expression induces apoptosis.J Cell Biol. 1998 May 4;141(3):741-53. doi: 10.1083/jcb.141.3.741. J Cell Biol. 1998. PMID: 9566973 Free PMC article.
-
Mechanism and regulation of eukaryotic protein synthesis.Microbiol Rev. 1992 Jun;56(2):291-315. doi: 10.1128/mr.56.2.291-315.1992. Microbiol Rev. 1992. PMID: 1620067 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
