Analysis of the biosynthesis of HLA-DR glycoproteins in human malignant melanoma cell lines
- PMID: 6785352
Analysis of the biosynthesis of HLA-DR glycoproteins in human malignant melanoma cell lines
Abstract
Human malignant melanoma cell line SK-MEL-37 expresses HLA-DR antigens having a characteristic 2-chain structure, with heavy chains (alpha) of approximately 32,000 daltons and light chains (beta) of approximately 28,000 daltons. Nonequilibrium pH-gradient electrophoresis (NEPHGE) on HLA-DR immunoprecipitated by rabbit anti-HLA-DR sera from [35S]-methionine-labeled, pulse-chased cells showed 2 closely spaced heavy-chain components (1, 2) and 4 light-chain spots (3-6). From nonchased samples, numerous more-basic components running in the heavy chain region were also precipitated. In particular, a very basic, 30,000 dalton component (pl approximately 8.5) was prominent (component 7); this spot probably corresponds to the invariant (Ii) peptide previously demonstrated in lymphoid cell HLA-DR precipitates (9) and the M-1 peptide of Shackelford and Strominger (10). None of these components (alpha, beta, or component 7) was precipitated from extracts of HLA-DR-negative melanoma cells. Pulse-chase experiments and the use of different labeled sugar precursors showed that component 7 is a partially glycosylated intracellular precursor, possibly of the HLA-DR alpha-chain. None of the immunoprecipitates, even from cells pulse-labeled for only 15 min, contained a peptide migrating in the 55,000 to 60,000 m.w. region. It was concluded that melanoma HLA-DR is not synthesized via a polyprotein precursor. In contrast to these results, obtained with rabbit anti-HLA-DR sera, a mouse monoclonal anti-HLA-DR was found to precipitate only biosynthetically completed alpha (1, 2) and beta (3-6) chains.
Similar articles
-
HLA-DR antigens of autologous melanoma and B lymphoblastoid cell lines: differences in glycosylation but not protein structure.J Immunol. 1984 Jul;133(1):315-20. J Immunol. 1984. PMID: 6609984
-
Two-dimensional gel analysis of the polypeptides precipitated by a polymorphic HLA-DR1,2,w6 monoclonal antibody: evidence for a third locus.Eur J Immunol. 1982 Jul;12(7):600-6. doi: 10.1002/eji.1830120713. Eur J Immunol. 1982. PMID: 6811289
-
Invariant chain associates with HLA class II antigens via its extracytoplasmic region.J Immunol. 1986 Apr 1;136(7):2519-25. J Immunol. 1986. PMID: 3512715
-
Analysis of DR-like molecules on a marmoset Epstein-Barr virus-induced cell line using a monomorphic anti-human HLA-DR monoclonal antibody.Eur J Immunol. 1982 May;12(5):446-8. doi: 10.1002/eji.1830120517. Eur J Immunol. 1982. PMID: 6284521
-
Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens.J Immunol. 1982 Dec;129(6):2564-9. J Immunol. 1982. PMID: 6982931
Cited by
-
Class 1 (unique) tumor antigens of human melanoma. Identification of a 90,000 dalton cell surface glycoprotein by autologous antibody.J Exp Med. 1984 Oct 1;160(4):1219-33. doi: 10.1084/jem.160.4.1219. J Exp Med. 1984. PMID: 6237165 Free PMC article.
-
Heterogeneity in surface antigen and glycoprotein expression of cell lines derived from different melanoma metastases of the same patient. Implications for the study of tumor antigens.J Exp Med. 1981 Dec 1;154(6):1764-78. doi: 10.1084/jem.154.6.1764. J Exp Med. 1981. PMID: 6976407 Free PMC article.
-
Beta-cell damage in diabetic insulitis: are we approaching a solution?Diabetologia. 1984 Apr;26(4):241-9. doi: 10.1007/BF00283644. Diabetologia. 1984. PMID: 6376230 No abstract available.
-
Integrin VLA-3: ultrastructural localization at cell-cell contact sites of human cell cultures.J Cell Biol. 1989 Oct;109(4 Pt 1):1807-15. doi: 10.1083/jcb.109.4.1807. J Cell Biol. 1989. PMID: 2677029 Free PMC article.
-
Human MHC class II molecules as differentiation markers.Immunogenetics. 1982;16(5):459-69. doi: 10.1007/BF00372104. Immunogenetics. 1982. PMID: 6984012
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Medical
Research Materials