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. 1980 Apr;77(4):1937-41.
doi: 10.1073/pnas.77.4.1937.

Transcription of mouse kappa chain genes: implications for allelic exclusion

R P Perry, D E Kelley, C Coleclough, J G Seidman, P Leder, S Tonegawa, G Matthyssens, M Weigert

Transcription of mouse kappa chain genes: implications for allelic exclusion

R P Perry et al. Proc Natl Acad Sci U S A. 1980 Apr.

Abstract

The nuclear RNA from a large variety of kappa-producing plasmacytomas was size fractionated and analyzed with a series of cloned probes representing sequences encoding variable (V), joining (J), and constant (C) regions and selected intervening sequences. All of the plasmacytomas produce a nuclear RNA component that contains V kappa and C kappa sequences as well as the intervening sequence between J kappa and C kappa, and that has a distinctive size depending on which of the four J kappa segments is expressed (i.e., is present in the secreted kappa chain). These RNAs are the precursors of kappa mRNAs, which are transcribed from productively rearranged C kappa genes. Half of the plasmacytomas examined produce, in addition to a kappa mRNA precursor, a discrete component of about 8.4 kilobases that contains C kappa and upstream flanking sequences but lacks the expressed V region sequence. The ability to produce this component is always associated with the persistence in the tumor genome of an unrearranged (germline) J kappa-C kappa region. In tumors rearranged at both kappa loci the nonproductive allele is either transcriptionally silent or, in a minority of cases, transcribed and processed into a "fragment" mRNA lacking V region sequences. These results reveal that allelic exclusion can be effected at several levels of gene expression. They also provide some insight into the relative contributions of the V and C gene elements to this expression.

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References

    1. Cell. 1975 Jun;5(2):139-47 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:877-89 - PubMed
    1. Cell. 1977 Oct;12(2):453-62 - PubMed
    1. Cell. 1977 Dec;12(4):1097-108 - PubMed

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