doi: 10.1042/bj2070617.
An enzyme-linked immunoassay for the collagen cross-link pyridinoline
- PMID: 6762208
- PMCID: PMC1153908
- DOI: 10.1042/bj2070617
Item in Clipboard
An enzyme-linked immunoassay for the collagen cross-link pyridinoline
Biochem J.
.
Abstract
An inhibition immunoassay method for the determination of pyridinoline was developed with the use of microtitre plates coated with a pyridinoline--gelatin conjugate and rabbit antisera directed against pyridinoline linked to bovine serum albumin. The sensitivity of the assay is about 2pmol of pyridinoline, and the presence of related pyridinium and lysine-derived compounds does not significantly interfere with the procedure. Its application to tissue and human urine samples is described.
Similar articles
-
An enzyme-linked immunosorbent assay to quantitate the elastin crosslink desmosine in tissue and urine samples.Anal Biochem. 1985 May 15;147(1):258-64. doi: 10.1016/0003-2697(85)90036-3. Anal Biochem. 1985. PMID: 4025822
-
Cross-linking of collagen. Location of pyridinoline in bovine articular cartilage at two sites of the molecule.Biochem J. 1983 Oct 1;215(1):175-82. doi: 10.1042/bj2150175. Biochem J. 1983. PMID: 6626173 Free PMC article.
-
An investigation of pyridinoline, and putative collagen cross-link.Biochem J. 1980 Feb 1;185(2):531-4. doi: 10.1042/bj1850531. Biochem J. 1980. PMID: 6772152 Free PMC article.
-
[Assay of cross-linking molecules of collagen (pyridinolines) in the study of the degradation of bone tissue and articular cartilage].Pathol Biol (Paris). 1993 Dec;41(10):951-61. Pathol Biol (Paris). 1993. PMID: 8159476 Review. French.
-
Measurement of the cross linking compound, pyridinoline, in urine as an index of collagen degradation in joint disease.Ann Rheum Dis. 1986 Dec;45(12):969-73. doi: 10.1136/ard.45.12.969. Ann Rheum Dis. 1986. PMID: 3813668 Free PMC article.
Cited by
-
Cross-linking in type IV collagen.Biochem J. 1984 Mar 15;218(3):713-23. doi: 10.1042/bj2180713. Biochem J. 1984. PMID: 6426460 Free PMC article.
-
Urinary excretion of pyridinium crosslinks of collagen in patients with osteoporosis and the effects of bone fracture.Ann Rheum Dis. 1992 May;51(5):648-51. doi: 10.1136/ard.51.5.648. Ann Rheum Dis. 1992. PMID: 1616331 Free PMC article.
-
Cross-linking of collagen. Isolation, structural characterization and glycosylation of pyridinoline.Biochem J. 1983 Oct 1;215(1):167-73. doi: 10.1042/bj2150167. Biochem J. 1983. PMID: 6626172 Free PMC article.
-
Preliminary results of the use of urinary excretion of pyridinium crosslinks for monitoring metastatic bone disease.Br J Cancer. 1992 May;65(5):766-8. doi: 10.1038/bjc.1992.161. Br J Cancer. 1992. PMID: 1586605 Free PMC article.
-
Pyridinium crosslinks as markers of bone resorption in patients with breast cancer.Br J Cancer. 1991 Nov;64(5):884-6. doi: 10.1038/bjc.1991.419. Br J Cancer. 1991. PMID: 1931610 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
