Motility and resorption: osteoclastic activity in vitro
- PMID: 6476408
- DOI: 10.1007/BF00319457
Motility and resorption: osteoclastic activity in vitro
Abstract
Rabbit osteoclasts (OCs), separated mechanically from long bones, were seeded on to glass or plastic substrates or slabs of sperm whale dentine (SWD). Cells were cultured in MEM with 10% FCS with or without added salmon calcitonin (SCT) at dosages of 0.001, 0.1 and 1 IU/ml. Although most rabbit SCT-treated OCs on the non-biological substrates showed inhibition of peripheral ruffling activity and motility at dosages that stop rat OC movement, resorption still occurred on the dentine. Thus such inhibition is unreliable as a general indicator for resorptive capability. Resorption lacunae were observed at all times from 6 h onwards. Using stereophotogrammetric techniques, the following minimum values were obtained from 24 h cultures: highest hourly rate of resorption of dentine for single OC, 570 micron3/h; average rate 165 micron3/h; mean total volume dentine removed per Howship's lacuna complex, 3,885 micron3; average value for plan area of surface attacked per OC, 1,450 micron2.
Similar articles
-
Variation in the sizes of resorption lacunae made in vitro.Scan Electron Microsc. 1986;(Pt 4):1571-80. Scan Electron Microsc. 1986. PMID: 3810027
-
Survival and resorptive activity of chick osteoclasts in culture.Anat Embryol (Berl). 1986;174(2):265-75. doi: 10.1007/BF00824342. Anat Embryol (Berl). 1986. PMID: 3740460
-
The effect of lipopolysaccharide from the oral bacterium Bacteroides gingivalis on osteoclastic resorption of sperm-whale dentine slices in vitro.Arch Oral Biol. 1987;32(12):911-3. doi: 10.1016/0003-9969(87)90106-3. Arch Oral Biol. 1987. PMID: 3503661
-
Biological aspects of altered bone remodeling in multiple myeloma and possibilities of pharmacological intervention.Dan Med Bull. 2011 May;58(5):B4277. Dan Med Bull. 2011. PMID: 21535989 Review.
-
Histomorphometry of Howship's lacunae formed in vivo and in vitro: depths and volumes measured by scanning electron and confocal microscopy.Bone. 1993 May-Jun;14(3):455-60. doi: 10.1016/8756-3282(93)90179-e. Bone. 1993. PMID: 8363892 Review. No abstract available.
Cited by
-
Monocyte-enriched cells on calcified tissues.Anat Embryol (Berl). 1984;170(2):169-75. doi: 10.1007/BF00319002. Anat Embryol (Berl). 1984. PMID: 6517352
-
A functional link between dynamin and the actin cytoskeleton at podosomes.J Cell Biol. 2000 Jul 24;150(2):377-89. doi: 10.1083/jcb.150.2.377. J Cell Biol. 2000. PMID: 10908579 Free PMC article.
-
Substrate influences rat osteoclast morphology and expression of potassium conductances.J Physiol. 1992 Dec;458:633-53. doi: 10.1113/jphysiol.1992.sp019438. J Physiol. 1992. PMID: 1338794 Free PMC article.
-
The complex rostral morphology and the endoskeleton ossification process of two adult samples of Xiphias gladius (Xiphiidae).J Fish Biol. 2022 Jul;101(1):42-54. doi: 10.1111/jfb.15069. Epub 2022 May 16. J Fish Biol. 2022. PMID: 35481825 Free PMC article.
-
The effect of fluoride on the patterns of adherence of osteoclasts cultured on and resorbing dentine: a 3-D assessment of vinculin-labelled cells using confocal optical microscopy.Anat Embryol (Berl). 1989;180(5):427-35. doi: 10.1007/BF00305117. Anat Embryol (Berl). 1989. PMID: 2515773