Clonal inheritance of the pattern of DNA methylation in mouse cells
- PMID: 6459581
- PMCID: PMC345661
- DOI: 10.1073/pnas.79.1.61
Clonal inheritance of the pattern of DNA methylation in mouse cells
Abstract
DNA-mediated gene transfer was used to investigate the mode of inheritance of 5-methylcytosine in mouse L cells. Unmethylated phi X174 replicative form DNA remains unmethylated after its introduction and integration into these cells. On the other hand, phi X174 replicative form DNA that was methylated in vitro at its C-C-G-G residues retains these methylations as shown by restriction enzyme analysis with Hpa II and Msp I to detect methylation at this specific site. Although these unselected methylated vectors are prone to lose 30-40% of their methyl moieties upon transfection, this demethylation appears to be random. Once established, the resulting methylation pattern is stable for at least 100 cell generations. In order to examine the specificity of methylation inheritance, fully hemimethylated duplex phi X174 DNA was synthesized in vitro from primed single-strand phi X174 DNA by using 5-methyl deoxycytidine 5'-triphosphate. This molecule was inserted into mouse L cells by cotransformation and subsequently was analyzed by a series of restriction enzymes. Only methylations located at C-G residues were conserved after many generations of cell growth. The results suggest that the inheritance of the cellular DNA methylation pattern is based on a C-G-specific methylase that operates on newly replicated hemimethylated DNA.
Similar articles
-
DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.J Virol. 1984 Mar;49(3):674-9. doi: 10.1128/JVI.49.3.674-679.1984. J Virol. 1984. PMID: 6321756 Free PMC article.
-
In vivo methylation of bacteriophage phi X174 DNA.J Virol. 1979 Dec;32(3):845-51. doi: 10.1128/JVI.32.3.845-851.1979. J Virol. 1979. PMID: 159962 Free PMC article.
-
Restriction enzyme digestion of hemimethylated DNA.Nucleic Acids Res. 1981 Jun 11;9(11):2509-15. doi: 10.1093/nar/9.11.2509. Nucleic Acids Res. 1981. PMID: 6269052 Free PMC article.
-
The flip side of DNA methylation.Cell. 1994 Jan 28;76(2):197-200. doi: 10.1016/0092-8674(94)90326-3. Cell. 1994. PMID: 8293456 Review. No abstract available.
-
Mammalian methyltransferases and methyl-CpG-binding domains: proteins involved in DNA methylation.Curr Top Microbiol Immunol. 2000;249:55-74. doi: 10.1007/978-3-642-59696-4_4. Curr Top Microbiol Immunol. 2000. PMID: 10802938 Review. No abstract available.
Cited by
-
DNA methyltransferase is developmentally expressed in replicating and non-replicating male germ cells.Nucleic Acids Res. 1992 May 25;20(10):2541-5. doi: 10.1093/nar/20.10.2541. Nucleic Acids Res. 1992. PMID: 1598212 Free PMC article.
-
Activation of mammalian DNA methyltransferase by cleavage of a Zn binding regulatory domain.EMBO J. 1992 Jul;11(7):2611-7. doi: 10.1002/j.1460-2075.1992.tb05326.x. EMBO J. 1992. PMID: 1628623 Free PMC article.
-
Introduction and expression of the bacterial PaeR7 methylase gene in mammalian cells.Proc Natl Acad Sci U S A. 1986 Oct;83(20):7713-7. doi: 10.1073/pnas.83.20.7713. Proc Natl Acad Sci U S A. 1986. PMID: 3463994 Free PMC article.
-
DNA methylation prevents the amplification of TROP1, a tumor-associated cell surface antigen gene.Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5833-7. doi: 10.1073/pnas.91.13.5833. Proc Natl Acad Sci U S A. 1994. PMID: 8016075 Free PMC article.
-
Induction and maintenance of nonsymmetrical DNA methylation in Neurospora.Proc Natl Acad Sci U S A. 2002 Dec 10;99 Suppl 4(Suppl 4):16485-90. doi: 10.1073/pnas.182427299. Epub 2002 Aug 20. Proc Natl Acad Sci U S A. 2002. PMID: 12189210 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
