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. 1981 Jan;145(1):288-92.
doi: 10.1128/jb.145.1.288-292.1981.

Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA

A Sarthy, S Michaelis, J Beckwith

Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA

A Sarthy et al. J Bacteriol. 1981 Jan.

Abstract

Lambda transducing phages containing portions of the phoA gene have been isolated and used to construct a deletion map of the phoA gene. The isolation of a plaque-forming lambda transducing phage carrying the entire phoA gene is also described. Two new methods for screening or selection of mutants that have altered levels of alkaline phosphatase activity are reported.

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