doi: 10.1111/j.1365-2818.1984.tb02513.x.
Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy
- PMID: 6429337
- DOI: 10.1111/j.1365-2818.1984.tb02513.x
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Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy
J Microsc.
1984 May.
Abstract
Cells of the bacterium Acholeplasma laidlawii were rapidly frozen against a copper block cooled by liquid helium. The frozen cells were transferred to liquid nitrogen and subsequently to acetone or methanol at 183 K. After 24 h the cells were infiltrated at 203 K with a non-polar methacrylate resin of the same type as Lowicryl HM20. The resin was cured at the same temperature. Acetone extracted approximately 5% of the lipid content of the cells, methanol 15-45% and the resin only negligible amounts. Similar results were obtained with A. laidlawii-ghosts. The cells appeared well preserved when examined in the electron microscope.
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