Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1984 Jan;4(1):92-100.
doi: 10.1128/mcb.4.1.92-100.1984.

Multiple L double-stranded RNA species of Saccharomyces cerevisiae: evidence for separate encapsidation

D J Thiele, E M Hannig, M J Leibowitz
Comparative Study

Multiple L double-stranded RNA species of Saccharomyces cerevisiae: evidence for separate encapsidation

D J Thiele et al. Mol Cell Biol. 1984 Jan.

Abstract

The L double-stranded (ds) RNA component of Saccharomyces cerevisiae may contain up to three dsRNA species, each with a distinct sequence but with identical molecular weights. These dsRNAs have been separated from each other by denaturation and polyacrylamide gel electrophoresis. The 3' terminal sequences of the major species, LA dsRNA, were determined. Secondary structural analysis supported the presence of two stem and loop structures at the 3' terminus of the LA positive strand. In strain T132B NK-3, both the LA and LC species are virion encapsidated. Two distinct classes of virions were purified from this strain, each with a different RNA polymerase activity and with distinct protein components. The heavy virions harbored LA dsRNA, whereas the LC dsRNA species co purified with the light virion peak. Thus, LA and LC dsRNAs, when present in the same cell, may be separately encapsidated.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1973 Sep 14;245(5420):81-6 - PubMed
    1. Nucleic Acids Res. 1980 Jun 11;8(11):2349-63 - PubMed
    1. Mol Cell Biol. 1983 Apr;3(4):654-61 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nucleic Acids Res. 1982 Mar 11;10(5):1661-78 - PubMed

Publication types

MeSH terms

LinkOut - more resources