Comparative Study
doi: 10.1128/mcb.4.1.86-91.1984.
Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae
- PMID: 6366514
- PMCID: PMC368661
- DOI: 10.1128/mcb.4.1.86-91.1984
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Comparative Study
Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae
Mol Cell Biol.
1984 Jan.
Abstract
We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.
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