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. 1983 Dec;35(2 Pt 1):521-9.
doi: 10.1016/0092-8674(83)90186-1.

Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor

D D Jenness, A C Burkholder, L H Hartwell

Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor

D D Jenness et al. Cell. 1983 Dec.

Abstract

The division cycle of yeast a cells is inhibited by alpha-factor. Haploid a cells were found to bind 35S-labeled alpha-factor, whereas haploid alpha cells and diploid a/alpha cells showed little binding. The association of alpha-factor with a cells was reversible upon dilution. Unlabeled alpha-factor competed for binding of 35S-alpha-factor; the concentration dependence for competition indicated 9 X 10(5) binding sites per cell with a dissociation constant (KD) of 3 X 10(-7) M. The rates of association (kon = 3 X 10(3) M-1 sec-1) and dissociation (koff = 9 X 10(-4) sec-1) were consistent with the equilibrium constant. The alpha-factor binding activity associated with five temperature-sensitive ste2 mutants was thermolabile, suggesting that the STE2 gene encodes the receptor for alpha-factor. In contrast, the binding activity of other temperature-sensitive mutants (ste4, ste5, ste7, ste11, and ste12) showed no thermolability.

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