doi: 10.1038/306557a0.
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes
- PMID: 6358900
- DOI: 10.1038/306557a0
Item in Clipboard
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes
Nature.
1983 Dec.
Abstract
DNA sequences containing the 5'-flanking regions of the insulin and chymotrypsin genes were linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. The insulin gene recombinant elicits preferential expression of CAT activity when introduced into cells producing insulin; similarly, the chymotrypsin gene recombinant elicits preferential expression in chymotrypsin-producing cells. Sequences located upstream of previously defined transcriptional control elements are essential for efficient expression in both cases.
Similar articles
-
Cell-specific enhancers in the rat exocrine pancreas.Proc Natl Acad Sci U S A. 1986 Jun;83(11):3599-603. doi: 10.1073/pnas.83.11.3599. Proc Natl Acad Sci U S A. 1986. PMID: 2424007 Free PMC article.
-
Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements.Science. 1985 Nov 22;230(4728):912-6. doi: 10.1126/science.3904002. Science. 1985. PMID: 3904002
-
Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia.Proc Natl Acad Sci U S A. 1985 Apr;82(8):2334-8. doi: 10.1073/pnas.82.8.2334. Proc Natl Acad Sci U S A. 1985. PMID: 3857584 Free PMC article.
-
Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression.Proc Natl Acad Sci U S A. 1983 Dec;80(24):7586-90. doi: 10.1073/pnas.80.24.7586. Proc Natl Acad Sci U S A. 1983. PMID: 6324179 Free PMC article.
-
1992 R.D. Lawrence Lecture. The regulation of insulin gene expression.Diabet Med. 1992 Nov;9(9):792-8. doi: 10.1111/j.1464-5491.1992.tb01896.x. Diabet Med. 1992. PMID: 1473318 Review. No abstract available.
Cited by
-
Distinct segments within the enhancer region collaborate to specify the type of leukemia induced by nondefective Friend and Moloney viruses.J Virol. 1989 Jan;63(1):328-37. doi: 10.1128/JVI.63.1.328-337.1989. J Virol. 1989. PMID: 2783259 Free PMC article.
-
Stable transfer and restricted expression of a cloned class I gene encoding a secreted transplantation-like antigen.Mol Cell Biol. 1985 Jun;5(6):1295-300. doi: 10.1128/mcb.5.6.1295-1300.1985. Mol Cell Biol. 1985. PMID: 4033655 Free PMC article.
-
DNA sequences responsible for tissue-specific expression of a chicken alpha-crystallin gene in mouse lens cells.EMBO J. 1985 Oct;4(10):2589-95. doi: 10.1002/j.1460-2075.1985.tb03975.x. EMBO J. 1985. PMID: 4054099 Free PMC article.
-
The cell-specific enhancer of the mouse transthyretin (prealbumin) gene binds a common factor at one site and a liver-specific factor(s) at two other sites.Mol Cell Biol. 1988 Jan;8(1):81-90. doi: 10.1128/mcb.8.1.81-90.1988. Mol Cell Biol. 1988. PMID: 3336368 Free PMC article.
-
Distribution and characterization of helix-loop-helix enhancer-binding proteins from pancreatic beta cells and lymphocytes.Nucleic Acids Res. 1991 Jul 25;19(14):3893-9. doi: 10.1093/nar/19.14.3893. Nucleic Acids Res. 1991. PMID: 1861981 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous
