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. 1983 Sep;34(2):477-90.
doi: 10.1016/0092-8674(83)90381-1.

Differential localization of two myosins within nematode thick filaments

D M Miller 3rd, I Ortiz, G C Berliner, H F Epstein

Differential localization of two myosins within nematode thick filaments

D M Miller 3rd et al. Cell. 1983 Sep.

Abstract

The body wall muscle cells of the nematode, Caenorhabditis elegans, contain two unique types of myosin heavy chain, A and B. We have utilized an immunochemical approach to define the structural location of these two myosins within body wall muscle thick filaments. By immunofluorescence microscopy, myosin B antibodies label the thick filament-containing A-bands of body wall muscle with the exception of a thin gap at the center of each A-band, and myosin A antibodies react to form a medial fluorescent stripe within each A-band. The complexes of these monoclonal antibodies with isolated thick filaments were negatively stained and studied by electron microscopy. The myosin B antibody reacts with the polar regions of all filaments but does not react with a central 0.9 micron zone. The myosin A antibody reacts with a central 1.8 micron zone in all filaments but does not react with the polar regions.

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