Immune response to human influenza virus hemagglutinin expressed in Escherichia coli
- PMID: 6343189
- DOI: 10.1016/0378-1119(83)90011-2
Immune response to human influenza virus hemagglutinin expressed in Escherichia coli
Abstract
Cloned DNA fragments coding for parts of strain WSN (H1N1) influenza virus hemagglutinin (HA) were fused to a bacterial leader DNA derived from the Escherichia coli trp operon. Fusion proteins produced consisted of 190 amino acids of trpLE' protein at the amino terminus, and HA amino acids, either 1-308, 1-396, or 1-548 (complete HA), at the carboxyl terminus. These proteins were expressed at high levels (10-20% of total protein) in E. coli starved for tryptophan. A CNBr fragment (HA1-211) was derived from HA-308. Each of the proteins was purified and used for immunizing mice and rabbits. The antibody produced was shown to bind to (i) the HA fusion proteins, (ii) detergent-treated viral HA, (iii) HA, on intact virions, and (iv) the HA on the surface of cells infected with influenza virus. This shows that the HA fusion proteins expressed in bacteria can elicit antibodies that recognize at least some determinants of the native viral HA, and probably could lead to development of an anti-influenza vaccine.
Similar articles
-
Biological and immunological properties of haemagglutinin and neuraminidase expressed from cloned cDNAs in prokaryotic and eukaryotic cells.Vaccine. 1985 Sep;3(3 Suppl):165-71. doi: 10.1016/0264-410x(85)90097-0. Vaccine. 1985. PMID: 2414936 Review.
-
Construction and characterization of a bacterial clone containing the hemagglutinin gene of the WSN strain (HON1) of influenza virus.Gene. 1980 Aug;10(3):205-18. doi: 10.1016/0378-1119(80)90050-5. Gene. 1980. PMID: 6254839
-
Cloned copy of the haemagglutinin gene codes for human influenza antigenic determinants in E. coli.Nature. 1981 Aug 27;292(5826):851-2. doi: 10.1038/292851a0. Nature. 1981. PMID: 6167865 No abstract available.
-
[Design of a recombinant strain of the vaccinia virus containing an expressible gene for influenza A virus hemagglutinin].Vopr Virusol. 1988 May-Jun;33(3):275-8. Vopr Virusol. 1988. PMID: 3176425 Russian.
-
Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli.Nature. 1980 Jan 10;283(5743):171-4. doi: 10.1038/283171a0. Nature. 1980. PMID: 6153123
Cited by
-
Signal processing, glycosylation, and secretion of mutant hemagglutinins of a human influenza virus by Saccharomyces cerevisiae.Mol Cell Biol. 1987 Apr;7(4):1476-85. doi: 10.1128/mcb.7.4.1476-1485.1987. Mol Cell Biol. 1987. PMID: 3037322 Free PMC article.
-
Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli.Proc Natl Acad Sci U S A. 1983 Oct;80(19):6105-9. doi: 10.1073/pnas.80.19.6105. Proc Natl Acad Sci U S A. 1983. PMID: 6310615 Free PMC article.
-
Formation of influenza virus particles lacking hemagglutinin on the viral envelope.J Virol. 1986 Dec;60(3):994-1001. doi: 10.1128/JVI.60.3.994-1001.1986. J Virol. 1986. PMID: 3783822 Free PMC article.
-
AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models.Virol J. 2020 Aug 5;17(1):121. doi: 10.1186/s12985-020-01388-w. Virol J. 2020. PMID: 32758272 Free PMC article.
-
Intracellular localization of the viral polymerase proteins in cells infected with influenza virus and cells expressing PB1 protein from cloned cDNA.J Virol. 1987 Jul;61(7):2217-24. doi: 10.1128/JVI.61.7.2217-2224.1987. J Virol. 1987. PMID: 2438429 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
