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. 1982;1(10):1213-6.
doi: 10.1002/j.1460-2075.1982.tb00015.x.

Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA

Y Wang, M Schäfer-Ridder, C Stratowa, T K Wong, P H Hofschneider

Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA

Y Wang et al. EMBO J. 1982.

Abstract

Hepatitis B virus (HBV) DNA was isolated from the recombinant plasmid pA01 -HBV and recircularized . Immediately after introduction of this DNA into mouse fibroblasts (NIH 3T3) we observed increasing release of hepatitis B surface antigen (HBsAg) into the culture medium. Later production of HBsAg declined to a lower but constant level. No dominant selective marker and foreign promoter were necessary in this system, which therefore can be used for the study of regulation of HBsAg expression.

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