The intranuclear location of a herpes simplex virus DNA-binding protein is determined by the status of viral DNA replication
- PMID: 6323024
- DOI: 10.1016/0092-8674(84)90035-7
The intranuclear location of a herpes simplex virus DNA-binding protein is determined by the status of viral DNA replication
Abstract
The herpes simplex viral DNA-binding protein, ICP8, is targeted to two different locations in the cell nucleus as part of its maturation pathway. Prior to viral DNA synthesis ICP8 was found at discrete pre-replicative sites throughout the nucleus, where it exhibited a high salt-labile association with the nuclear matrix. During viral DNA replication ICP8 was localized in randomly distributed replication compartments, where it is bound to viral DNA. Initiation of viral DNA replication caused the protein to move from the prereplicative sites to the replication compartments, while inhibition of replication caused movement in the opposite direction. In cells where viral DNA synthesis was proceeding, a minor population of ICP8 may also have been associated with the prereplicative sites. The prereplicative sites may serve as a nuclear reservoir for ICP8 not bound to replicating or progeny DNA.
Similar articles
-
Correct intranuclear localization of herpes simplex virus DNA polymerase requires the viral ICP8 DNA-binding protein.J Virol. 1991 Mar;65(3):1082-9. doi: 10.1128/JVI.65.3.1082-1089.1991. J Virol. 1991. PMID: 1847437 Free PMC article.
-
C-terminal region of herpes simplex virus ICP8 protein needed for intranuclear localization.Virology. 2003 May 10;309(2):219-31. doi: 10.1016/s0042-6822(03)00108-9. Virology. 2003. PMID: 12758170
-
Herpes simplex virus replication compartments can form by coalescence of smaller compartments.Virology. 2003 May 10;309(2):232-47. doi: 10.1016/s0042-6822(03)00107-7. Virology. 2003. PMID: 12758171
-
ICP8 Filament Formation Is Essential for Replication Compartment Formation during Herpes Simplex Virus Infection.J Virol. 2015 Dec 16;90(5):2561-70. doi: 10.1128/JVI.02854-15. J Virol. 2015. PMID: 26676794 Free PMC article.
-
Replication Compartments of DNA Viruses in the Nucleus: Location, Location, Location.Viruses. 2020 Jan 29;12(2):151. doi: 10.3390/v12020151. Viruses. 2020. PMID: 32013091 Free PMC article. Review.
Cited by
-
Visualizing the replicating HSV-1 virus using STED super-resolution microscopy.Virol J. 2016 Apr 9;13:65. doi: 10.1186/s12985-016-0521-7. Virol J. 2016. PMID: 27062411 Free PMC article.
-
Kinetics of expression of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.J Virol. 1989 Jan;63(1):137-47. doi: 10.1128/JVI.63.1.137-147.1989. J Virol. 1989. PMID: 2535721 Free PMC article.
-
Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression.J Virol. 1991 May;65(5):2666-75. doi: 10.1128/JVI.65.5.2666-2675.1991. J Virol. 1991. PMID: 1850040 Free PMC article.
-
Identification and functional evaluation of cellular and viral factors involved in the alteration of nuclear architecture during herpes simplex virus 1 infection.J Virol. 2005 Oct;79(20):12840-51. doi: 10.1128/JVI.79.20.12840-12851.2005. J Virol. 2005. PMID: 16188986 Free PMC article.
-
Inhibition of the herpes simplex virus type 1 DNA polymerase induces hyperphosphorylation of replication protein A and its accumulation at S-phase-specific sites of DNA damage during infection.J Virol. 2005 Jun;79(11):7162-71. doi: 10.1128/JVI.79.11.7162-7171.2005. J Virol. 2005. PMID: 15890955 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
