The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA
- PMID: 6317188
- DOI: 10.1016/0092-8674(83)90176-9
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA
Abstract
We have studied the exact sequence requirement for the formation of 3' termini of the sea urchin H2A mRNA in frog oocyte injection experiments. Point mutations destroying the symmetry of the inverted DNA repeat in the mRNA trailer coding sequences prevent the generation of genuine 3' termini. Mutants containing complementary base changes are pseudorevertants and allow the production of H2A mRNA with faithful 3' termini at wild-type levels. Our transcription analyses show that it is primarily the sequence of the transcribed strand that decides whether or not true 3' mRNA termini are produced. This is evidence that an RNA stem-loop structure, rather than a DNA cruciform, is essential for this process. Spacer sequences are absolutely required, because in their absence only H2A mRNA with spacer transcript extensions are found. Once the canonical CAAGAAAGA and flanking sequences are linked to the H2A gene, H2A messenger is synthesized at a suboptimal rate, which can be increased to wild-type levels by the addition of 80 bp of the spacer immediately adjacent to the H2A gene.
Similar articles
-
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.Cell. 1982 Apr;28(4):739-45. doi: 10.1016/0092-8674(82)90053-8. Cell. 1982. PMID: 6284372
-
The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processing.EMBO J. 1985 Feb;4(2):481-9. doi: 10.1002/j.1460-2075.1985.tb03654.x. EMBO J. 1985. PMID: 2410259 Free PMC article.
-
3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.Proc Natl Acad Sci U S A. 1984 Feb;81(4):1057-61. doi: 10.1073/pnas.81.4.1057. Proc Natl Acad Sci U S A. 1984. PMID: 6583695 Free PMC article.
-
The modulator is a constitutive enhancer of a developmentally regulated sea urchin histone H2A gene.Bioessays. 2002 Sep;24(9):850-7. doi: 10.1002/bies.10143. Bioessays. 2002. PMID: 12210522 Review.
-
Evolutionary aspects of cell differentiation.Int Rev Cytol. 1982;75:229-41. doi: 10.1016/s0074-7696(08)61006-2. Int Rev Cytol. 1982. PMID: 6179896 Review. No abstract available.
Cited by
-
Sequences of four mouse histone H3 genes: implications for evolution of mouse histone genes.J Mol Evol. 1986;23(3):242-9. doi: 10.1007/BF02115580. J Mol Evol. 1986. PMID: 3027355
-
Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.Mol Cell Biol. 1985 May;5(5):1143-50. doi: 10.1128/mcb.5.5.1143-1150.1985. Mol Cell Biol. 1985. PMID: 2582239 Free PMC article.
-
Globin gene transcripts can utilize histone gene 3' end processing signals.Nucleic Acids Res. 1986 Sep 11;14(17):7059-70. doi: 10.1093/nar/14.17.7059. Nucleic Acids Res. 1986. PMID: 3463933 Free PMC article.
-
An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.Mol Cell Biol. 1991 Jan;11(1):497-509. doi: 10.1128/mcb.11.1.497-509.1991. Mol Cell Biol. 1991. PMID: 1986243 Free PMC article.
-
A signal regulating mouse histone H4 mRNA levels in a mammalian cell cycle mutant and sequences controlling RNA 3' processing are both contained within the same 80-bp fragment.EMBO J. 1986 Dec 1;5(12):3297-303. doi: 10.1002/j.1460-2075.1986.tb04643.x. EMBO J. 1986. PMID: 3816761 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous
