Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes
- PMID: 6309857
- PMCID: PMC2112509
- DOI: 10.1083/jcb.97.2.329
Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes
Abstract
At 4 degrees C transferrin bound to receptors on the reticulocyte plasma membrane, and at 37 degrees C receptor-mediated endocytosis of transferrin occurred. Uptake at 37 degrees C exceeded binding at 4 degrees C by 2.5-fold and saturated after 20-30 min. During uptake at 37 degrees C, bound transferrin was internalized into a trypsin-resistant space. Trypsinization at 4 degrees C destroyed surface receptors, but with subsequent incubation at 37 degrees C, surface receptors rapidly appeared (albeit in reduced numbers), and uptake occurred at a decreased level. After endocytosis, transferrin was released, apparently intact, into the extracellular space. At 37 degrees C colloidal gold-transferrin (AuTf) clustered in coated pits and then appeared inside various intracellular membrane-bounded compartments. Small vesicles and tubules were labeled after short (5-10 min) incubations at 37 degrees C. Larger multivesicular endosomes became heavily labeled after longer (20-35 min) incubations. Multivesicular endosomes apparently fused with the plasma membrane and released their contents by exocytosis. None of these organelles appeared to be lysosomal in nature, and 98% of intracellular AuTf was localized in acid phosphatase-negative compartments. AuTf, like transferrin, was released with subsequent incubation at 37 degrees C. Freeze-dried and freeze-fractured reticulocytes confirmed the distribution of AuTf in reticulocytes and revealed the presence of clathrin-coated patches amidst the spectrin coating the inner surface of the plasma membrane. These data suggest that transferrin is internalized via coated pits and vesicles and demonstrate that transferrin and its receptor are recycled back to the plasma membrane after endocytosis.
Similar articles
-
Receptor-mediated endocytosis of transferrin and ferritin by guinea-pig reticulocytes. Uptake by a common endocytic pathway.Eur J Cell Biol. 1987 Apr;43(2):260-5. Eur J Cell Biol. 1987. PMID: 3036529
-
Endocytosis and intracellular processing of transferrin and colloidal gold-transferrin in rat reticulocytes: demonstration of a pathway for receptor shedding.Eur J Cell Biol. 1984 Nov;35(2):256-63. Eur J Cell Biol. 1984. PMID: 6151502
-
Transferrin and ferritin endocytosis and recycling in guinea-pig reticulocytes.Biochim Biophys Acta. 1987 Jun 15;929(1):18-24. doi: 10.1016/0167-4889(87)90236-9. Biochim Biophys Acta. 1987. PMID: 3036246
-
Receptor-mediated endocytosis: the intracellular journey of transferrin and its receptor.Biochimie. 1986 Mar;68(3):375-81. doi: 10.1016/s0300-9084(86)80004-9. Biochimie. 1986. PMID: 2874839 Review.
-
Sorting and recycling of cell surface receptors and endocytosed ligands: the asialoglycoprotein and transferrin receptors.J Cell Biochem. 1983;23(1-4):107-30. doi: 10.1002/jcb.240230111. J Cell Biochem. 1983. PMID: 6327736 Review.
Cited by
-
Ubiquitin-driven protein condensation stabilizes clathrin-mediated endocytosis.PNAS Nexus. 2024 Aug 21;3(9):pgae342. doi: 10.1093/pnasnexus/pgae342. eCollection 2024 Sep. PNAS Nexus. 2024. PMID: 39253396 Free PMC article.
-
Intercellular Molecular Transfer Mediated by Extracellular Vesicles in Cancer.Results Probl Cell Differ. 2024;73:327-352. doi: 10.1007/978-3-031-62036-2_14. Results Probl Cell Differ. 2024. PMID: 39242385 Review.
-
Isolation and Characterization of Extracellular Vesicles to Activate Retina Regeneration.Methods Mol Biol. 2025;2848:135-150. doi: 10.1007/978-1-0716-4087-6_9. Methods Mol Biol. 2025. PMID: 39240521
-
Exosomal misfolded proteins released by cancer stem cells: dual functions in balancing protein homeostasis and orchestrating tumor progression.Discov Oncol. 2024 Aug 31;15(1):392. doi: 10.1007/s12672-024-01262-z. Discov Oncol. 2024. PMID: 39215782 Free PMC article. Review.
-
Role of Extracellular Vesicles in the Progression of Brain Tumors.Biology (Basel). 2024 Aug 2;13(8):586. doi: 10.3390/biology13080586. Biology (Basel). 2024. PMID: 39194524 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources