Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein
- PMID: 6300434
- PMCID: PMC256472
- DOI: 10.1128/JVI.45.2.773-781.1983
Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein
Abstract
A cDNA clone containing the entire vesicular stomatitis virus nucleocapsid gene was assembled by fusing portions of two partial clones. When the cDNA clone was inserted into a new general-purpose eucaryotic expression vector and introduced into appropriate host cells, abundant N-protein synthesis ensued. The expressed protein was indistinguishable from authentic N protein produced during vesicular stomatitis virus infections. The recombinant N protein was recognized by a polyclonal antibody and two different monoclonal antibodies and could not be resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis from authentic N. Our results suggest that the recombinant N protein produced in transfected cells rapidly aggregates into high-molecular-weight complexes in the absence of vesicular stomatitis virus genomic RNA.
Similar articles
-
Expression of the vesicular stomatitis virus nucleocapsid protein gene in Escherichia coli: analysis of its biological activity in vitro.Virology. 1993 Mar;193(1):340-7. doi: 10.1006/viro.1993.1130. Virology. 1993. PMID: 8382400
-
Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells.Cell. 1982 Oct;30(3):753-62. doi: 10.1016/0092-8674(82)90280-x. Cell. 1982. PMID: 6291783
-
Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli.Proc Natl Acad Sci U S A. 1981 Nov;78(11):6670-4. doi: 10.1073/pnas.78.11.6670. Proc Natl Acad Sci U S A. 1981. PMID: 6273881 Free PMC article.
-
The nucleocapsid of vesicular stomatitis virus.Sci China Life Sci. 2012 Apr;55(4):291-300. doi: 10.1007/s11427-012-4307-x. Epub 2012 May 9. Sci China Life Sci. 2012. PMID: 22566085 Review.
-
Gene expression of vesicular stomatitis virus genome RNA.Virology. 1992 Jun;188(2):417-28. doi: 10.1016/0042-6822(92)90495-b. Virology. 1992. PMID: 1316668 Review. No abstract available.
Cited by
-
Location of the binding domains for the RNA polymerase L and the ribonucleocapsid template within different halves of the NS phosphoprotein of vesicular stomatitis virus.Proc Natl Acad Sci U S A. 1987 Aug;84(16):5655-9. doi: 10.1073/pnas.84.16.5655. Proc Natl Acad Sci U S A. 1987. PMID: 2441389 Free PMC article.
-
mRNA poly(A) tail, a 3' enhancer of translational initiation.Mol Cell Biol. 1990 Jul;10(7):3441-55. doi: 10.1128/mcb.10.7.3441-3455.1990. Mol Cell Biol. 1990. PMID: 1972543 Free PMC article.
-
Characterization of mouse myelin basic protein messenger RNAs with a myelin basic protein cDNA clone.Proc Natl Acad Sci U S A. 1984 Jan;81(1):18-22. doi: 10.1073/pnas.81.1.18. Proc Natl Acad Sci U S A. 1984. PMID: 6198644 Free PMC article.
-
A single amino acid substitution in the variable region of the light chain specifically blocks immunoglobulin secretion.Proc Natl Acad Sci U S A. 1990 Oct;87(20):8135-9. doi: 10.1073/pnas.87.20.8135. Proc Natl Acad Sci U S A. 1990. PMID: 2122454 Free PMC article.
-
A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein.J Cell Biol. 1991 Oct;115(1):19-30. doi: 10.1083/jcb.115.1.19. J Cell Biol. 1991. PMID: 1655802 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
