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. 1983 Jan;45(1):332-42.
doi: 10.1128/JVI.45.1.332-342.1983.

Characterization of herpes simplex virus 2 temperature-sensitive mutants whose lesions map in or near the coding sequences for the major DNA-binding protein

A E Spang, P J Godowski, D M Knipe

Characterization of herpes simplex virus 2 temperature-sensitive mutants whose lesions map in or near the coding sequences for the major DNA-binding protein

A E Spang et al. J Virol. 1983 Jan.

Abstract

By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected with certain of these mutants at the nonpermissive temperature, the association of the major DNA-binding protein with the cell nucleus was defective. In these cells, the DNA-binding protein accumulated in the cytoplasmic and the crude nuclear detergent wash fractions. At the permissive temperature, the maturation of the mutant ICP8 was similar to that of the wild-type viral protein. With the remainder of the mutants, the nuclear maturation of ICP8 was similar to that encoded by the wild-type virus at the nonpermissive and permissive temperatures as assayed by cell fractionation.

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References

    1. J Virol. 1981 Jan;37(1):191-206 - PubMed
    1. J Virol. 1980 Jan;33(1):167-82 - PubMed
    1. J Virol. 1981 Sep;39(3):894-902 - PubMed
    1. J Mol Biol. 1981 Jul 25;150(1):15-32 - PubMed
    1. J Virol. 1982 Jul;43(1):314-24 - PubMed

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