doi: 10.1126/science.6283640.
Active genes are sensitive to deoxyribonuclease I during metaphase
- PMID: 6283640
- DOI: 10.1126/science.6283640
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Active genes are sensitive to deoxyribonuclease I during metaphase
Science.
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Abstract
The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.
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