doi: 10.1128/jb.149.1.407-410.1982.
Amino-terminal sequence of the Tn3 transposase protein
- PMID: 6274848
- PMCID: PMC216644
- DOI: 10.1128/jb.149.1.407-410.1982
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Amino-terminal sequence of the Tn3 transposase protein
J Bacteriol.
1982 Jan.
Abstract
The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.
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