Biosynthesis of the human transferrin receptor in cultured cells
- PMID: 6273413
Biosynthesis of the human transferrin receptor in cultured cells
Abstract
The biosynthesis and degradation of the cell surface transferrin receptor has been investigated. The receptor is a glycoprotein, and evidence is presented that the mature receptor contains both complex and high mannose N-asparagine-linked oligosaccharides that are synthesized via a common high mannose intermediate as previously described for other glycoproteins. It is shown that fatty acid is associated with only the mature form of the receptor and that addition of fatty acid to the receptor and that addition of fatty acid to the receptor can occur as long as 48 h after synthesis. Glycosylation is not an absolute requirement for the receptor to act as acceptor for fatty acid, nor for transport to the cell surface, although the efficiency of both processes may be reduced in tunicamycin-treated cells. The protein moiety of the transferrin receptor is degraded with a half-life of approximately 60 h.
Similar articles
-
Characterization of the transferrin receptor in tunicamycin-treated A431 cells.J Biol Chem. 1988 May 25;263(15):7297-301. J Biol Chem. 1988. PMID: 3366781
-
Structural features of the cell surface receptor for transferrin that is recognized by the monoclonal antibody OKT9.J Biol Chem. 1982 Jul 25;257(14):8516-22. J Biol Chem. 1982. PMID: 6282884
-
Biosynthesis of the insulin-like growth factor-II (IGF-II)/mannose-6-phosphate receptor in rat C6 glial cells: the role of N-linked glycosylation in binding of IGF-II to the receptor.Mol Endocrinol. 1991 Feb;5(2):281-91. doi: 10.1210/mend-5-2-281. Mol Endocrinol. 1991. PMID: 1645456
-
Effect of glycosylation inhibitors on the structure and function of the murine transferrin receptor.Eur J Biochem. 1989 Dec 22;186(3):637-47. doi: 10.1111/j.1432-1033.1989.tb15254.x. Eur J Biochem. 1989. PMID: 2514095
-
Inhibitors of the biosynthesis and processing of N-linked oligosaccharides.CRC Crit Rev Biochem. 1984;16(1):21-49. doi: 10.3109/10409238409102805. CRC Crit Rev Biochem. 1984. PMID: 6232113 Review.
Cited by
-
Effect of cycloheximide on palmitylation of PO protein of the peripheral nervous system myelin.Biochem J. 1989 Oct 1;263(1):173-7. doi: 10.1042/bj2630173. Biochem J. 1989. PMID: 2481437 Free PMC article.
-
Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells.J Cell Biol. 1983 Aug;97(2):508-21. doi: 10.1083/jcb.97.2.508. J Cell Biol. 1983. PMID: 6309862 Free PMC article.
-
Transferrin receptors on lymphocytes: structure, expression, evolution and function.Surv Immunol Res. 1983;2(2):129-36. doi: 10.1007/BF02918570. Surv Immunol Res. 1983. PMID: 6316457 Review. No abstract available.
-
Biogenesis of the polymeric IgA receptor in rat hepatocytes. I. Kinetic studies of its intracellular forms.J Cell Biol. 1985 Apr;100(4):1248-54. doi: 10.1083/jcb.100.4.1248. J Cell Biol. 1985. PMID: 3920224 Free PMC article.
-
The anti-tumour agent, cisplatin, and its clinically ineffective isomer, transplatin, produce unique gene expression profiles in human cells.Cancer Inform. 2008;6:315-55. doi: 10.4137/cin.s802. Epub 2008 Jun 10. Cancer Inform. 2008. PMID: 19259415 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
