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. 1980 May;34(2):550-9.
doi: 10.1128/JVI.34.2.550-559.1980.

Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA

M Schubert, J D Keene, R C Herman, R A Lazzarini

Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA

M Schubert et al. J Virol. 1980 May.

Abstract

The 5'-terminal nucleotide sequence from positions 50 to 130 of vesicular stomatitis virus RNA was determined indirectly by using a defective interfering particle RNA which contains covalently linked genomic minus and antigenomic plus sense RNAs. The last 18 nucleotides of the L gene coding for in the viral polymerase were identified and isolated by specific duplex formation between 5' terminally labeled oligonucleotides from a small single-stranded defective interfering particle RNA and L gene mRNA. The L gene ends at position 60 from the 5' terminus of the vesicular stomatitis genome. The data demonstrated that the first seven adenine residues in the polyadenylic acid tail of L gene mRNA may be coded for in the genome and suggested that the viral transcriptase itself may carry out polyadenylation, possibly by chattering at the uridine-rich sequence at the end of the L gene. Analysis of the 5'-terminal sequence of vesicular stomatitis virus genomic RNA revealed that it might fold into a complex secondary structure with possibly 62% of the bases paired.

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