Purification and characterization of a Saccharomyces cerevisiae exoribonuclease which yields 5'-mononucleotides by a 5' leads to 3' mode of hydrolysis
- PMID: 6244307
Purification and characterization of a Saccharomyces cerevisiae exoribonuclease which yields 5'-mononucleotides by a 5' leads to 3' mode of hydrolysis
Abstract
An exoribonuclease producing 5'-mononucleotides has been purified from ribosomes of Saccharomyces cerevisiae. The enzyme has a broad pH optimum around 8.0, requires divalent cation, and is stimulated by monovalent cation with the cation and degree of stimulation being dependent on the substrate used. With either poly(A) or rRNA as substrate, the enzyme has a processive mode of hydrolysis. The oligonucleotides, (pA)3-5, are hydrolyzed by the enzyme, and the hydrolysis is dependent on a 5'-phosphate end group. Phosphorylation of the 3' end has little effect on the rate of hydrolysis. With [3H]poly(A) or [3H]rRNA, labeled differentially at the 5' termini, a more rapid release of 5'-terminal label can be shown, providing evidence that the enzyme hydrolyzes in a 5' leads to 3' direction. Further evidence for a 5' leads to 3' mode of hydrolysis is provided by a study of the products of the hydrolysis of [3H](pA)5 labeled at the 5' termini with 32P. No 32P label is found in (pA)2 which accumulates as an intermediate.
Similar articles
-
5'-exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5'-exonuclease-1.J Biol Chem. 1995 Jul 7;270(27):16063-9. doi: 10.1074/jbc.270.27.16063. J Biol Chem. 1995. PMID: 7608167
-
An exoribonuclease from Saccharomyces cerevisiae: effect of modifications of 5' end groups on the hydrolysis of substrates to 5' mononucleotides.Biochem Biophys Res Commun. 1978 Mar 30;81(2):656-61. doi: 10.1016/0006-291x(78)91586-3. Biochem Biophys Res Commun. 1978. PMID: 352351 No abstract available.
-
Purification and characterization of a poly(A)-specific exoribonuclease from calf thymus.J Biol Chem. 1980 May 25;255(10):4535-8. J Biol Chem. 1980. PMID: 6246077
-
Exonuclease II from Saccharomyces cerevisiae. An enzyme which liberates 5'-deoxyribomononucleotides from single-stranded DNA by a 5' goes to 3' mode of hydrolysis.J Biol Chem. 1982 Jul 25;257(14):8177-82. J Biol Chem. 1982. PMID: 6282877
-
Purification and mode of action of exoribonuclease from bovine brain.J Biol Chem. 1977 Sep 25;252(18):6416-20. J Biol Chem. 1977. PMID: 893414
Cited by
-
Competing and noncompeting activities of miR-122 and the 5' exonuclease Xrn1 in regulation of hepatitis C virus replication.Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1881-6. doi: 10.1073/pnas.1213515110. Epub 2012 Dec 17. Proc Natl Acad Sci U S A. 2013. PMID: 23248316 Free PMC article.
-
LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.Mol Biol Cell. 2012 Feb;23(4):716-28. doi: 10.1091/mbc.E11-06-0530. Epub 2011 Dec 21. Mol Biol Cell. 2012. PMID: 22190735 Free PMC article.
-
DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs.Genes Dev. 2016 Sep 15;30(18):2076-2092. doi: 10.1101/gad.282616.116. Genes Dev. 2016. PMID: 27798849 Free PMC article.
-
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry.J Vis Exp. 2022 Apr 11;(182):10.3791/63720. doi: 10.3791/63720. J Vis Exp. 2022. PMID: 35467656 Free PMC article.
-
Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I.EMBO Rep. 2010 Oct;11(10):758-64. doi: 10.1038/embor.2010.130. Epub 2010 Sep 3. EMBO Rep. 2010. PMID: 20814424 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
