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. 1984 Aug;81(15):4819-23.
doi: 10.1073/pnas.81.15.4819.

Two nuclear mutations that block mitochondrial protein import in yeast

M P Yaffe, G Schatz

Two nuclear mutations that block mitochondrial protein import in yeast

M P Yaffe et al. Proc Natl Acad Sci U S A. 1984 Aug.

Abstract

We isolated two yeast mutants that are temperature-sensitive for import of mitochondrial proteins. Each strain contains a single mutation that results in arrest of growth and accumulation of precursor to the beta subunit of the mitochondrial F1-ATPase after incubation at 37 degrees C. These lesions (mas1 and mas2) are nonallelic and recessive. Cells harboring either mutation stop growing only after 2-3 generations at 37 degrees C. Import of the F1 beta subunit at 37 degrees C is more than 250 times slower in mas1 and 15 times slower in mas2 than in wild-type cells. At 23 degrees C, import occurs with similar rates in mutant and wild-type cells. The two mutations also reduce the rate of import of other proteins; however, import of different precursors is affected to different degrees in the two strains. The temperature-sensitive step in import in both mas1 and mas2 occurs before arrival of precursors in the mitochondrial matrix.

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References

    1. Anal Biochem. 1979 Sep 15;98(1):132-5 - PubMed
    1. Eur J Biochem. 1982 Jun 15;125(1):109-16 - PubMed
    1. J Biol Chem. 1982 Nov 10;257(21):13056-61 - PubMed
    1. J Biol Chem. 1982 Nov 10;257(21):13034-41 - PubMed
    1. J Biol Chem. 1983 Jun 10;258(11):6671-4 - PubMed

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