A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA
- PMID: 6200938
- DOI: 10.1126/science.6200938
A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA
Abstract
The excised intervening sequence of the Tetrahymena ribosomal RNA precursor mediates its own covalent cyclization in the absence of any protein. The circular molecule undergoes slow reopening at a single phosphodiester bond, the one that was formed during cyclization. The resulting linear molecule has 5'-phosphate and 3'-hydroxyl termini; these are unusual products for RNA hydrolysis but are typical of the other reactions mediated by this molecule. The reopened circle retains cleavage-ligation activity, as evidenced by its ability to undergo another round of cyclization and reopening. The finding that an RNA molecule can be folded so that a specific phosphate can be strained or activated helps to explain how the activation energy is lowered for RNA self-splicing. The proposed mechanisms may be relevant to several other RNA cleavage reactions that are RNA-mediated.
Similar articles
-
Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage-ligation reaction.Nature. 1983 Feb 17-23;301(5901):578-83. doi: 10.1038/301578a0. Nature. 1983. PMID: 6186917
-
Reactions of the intervening sequence of the Tetrahymena ribosomal ribonucleic acid precursor: pH dependence of cyclization and site-specific hydrolysis.Biochemistry. 1985 Oct 22;24(22):6211-8. doi: 10.1021/bi00343a027. Biochemistry. 1985. PMID: 4084515
-
New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicing.J Mol Biol. 1986 May 5;189(1):143-65. doi: 10.1016/0022-2836(86)90387-6. J Mol Biol. 1986. PMID: 2431151
-
RNA as an enzyme.Biochem Int. 1989 Jan;18(1):7-14. Biochem Int. 1989. PMID: 2470377 Review.
-
Nobel lecture. Self-splicing and enzymatic activity of an intervening sequence RNA from Tetrahymena.Biosci Rep. 1990 Jun;10(3):239-61. doi: 10.1007/BF01117241. Biosci Rep. 1990. PMID: 1699616 Review.
Cited by
-
Site-specific RNA crosslinking with 4-thiouridine.Mol Biol Rep. 1994 Jul;20(1):35-44. doi: 10.1007/BF00999853. Mol Biol Rep. 1994. PMID: 7531281 Review. No abstract available.
-
Mutational specificity of a bacteriophage T4 DNA polymerase mutant, mel88.Mol Gen Genet. 1987 Aug;209(1):90-3. doi: 10.1007/BF00329841. Mol Gen Genet. 1987. PMID: 3312960
-
Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena: inhibition by intercalating dyes.Nucleic Acids Res. 1985 Nov 11;13(21):7741-58. doi: 10.1093/nar/13.21.7741. Nucleic Acids Res. 1985. PMID: 2415923 Free PMC article.
-
Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway.Proc Natl Acad Sci U S A. 1986 Aug;83(16):5875-9. doi: 10.1073/pnas.83.16.5875. Proc Natl Acad Sci U S A. 1986. PMID: 3526343 Free PMC article.
-
Sub-3-Å cryo-EM structure of RNA enabled by engineered homomeric self-assembly.Nat Methods. 2022 May;19(5):576-585. doi: 10.1038/s41592-022-01455-w. Epub 2022 May 2. Nat Methods. 2022. PMID: 35501384
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
