Membrane biogenesis in the sprouting neuron. I. Selective transfer of newly synthesized phospholipid into the growing neurite
- PMID: 6194160
- PMCID: PMC2112594
- DOI: 10.1083/jcb.97.4.1038
Membrane biogenesis in the sprouting neuron. I. Selective transfer of newly synthesized phospholipid into the growing neurite
Abstract
Our goal was to elucidate the pathway of newly synthesized phospholipid into the growing neurite. This was accomplished in pulse-chase studies with the phospholipid precursor [3H]glycerol, using sprouting explant cultures of rat superior cervical ganglion as an experimental system. After the pulse with the precursor and various chase periods, we separated perikarya and neurites microsurgically and extracted their phospholipids. The phospholipid extract from the perikarya exhibited a steep rise followed by a rapid decline in specific radioactivity. In contrast, an increase in neuritic specific radioactivity of phospholipid was observed only after a lag period of approximately 60 min. Nearly quantitative transfer of newly synthesized phospholipid from the perikarya into the neurites could be demonstrated. Both the decline in perikaryal specific radioactivity and the increase in its neuritic counterpart, i.e., the proximodistal transfer, could be blocked with the microtubule drug colchicine and the metabolic uncoupler, 2,4-dinitrophenol. These observations indicate preferential export of newly synthesized phospholipid from the perikaryon (the major or exclusive site of synthesis) into the growing neurites, most likely by rapid axoplasmic transport of formed elements.
Similar articles
-
Nerve growth factor stimulates phospholipid methylation in growing neurites.Proc Natl Acad Sci U S A. 1981 Dec;78(12):7797-800. doi: 10.1073/pnas.78.12.7797. Proc Natl Acad Sci U S A. 1981. PMID: 6950418 Free PMC article.
-
Selective retrograde axonal transport of free glycine in identified neurons of Aplysia.Cell Mol Neurobiol. 1984 Sep;4(3):231-47. doi: 10.1007/BF00733587. Cell Mol Neurobiol. 1984. PMID: 6084551
-
Delivery of newly synthesized tubulin to rapidly growing distal axons of sympathetic neurons in compartmented cultures.J Cell Biol. 1996 Nov;135(3):701-9. doi: 10.1083/jcb.135.3.701. J Cell Biol. 1996. PMID: 8909544 Free PMC article.
-
Axons of sympathetic neurons; transport of enzymes in vivo and properties of axonal sprouts in vitro.Pharmacol Rev. 1972 Jun;24(2):245-54. Pharmacol Rev. 1972. PMID: 4117969 Review. No abstract available.
-
Relationships between the rapid axonal transport of newly synthesized proteins and membranous organelles.Mol Neurobiol. 1992 Summer-Fall;6(2-3):285-300. doi: 10.1007/BF02780558. Mol Neurobiol. 1992. PMID: 1282335 Review.
Cited by
-
D1-type dopamine receptors inhibit growth cone motility in cultured retina neurons: evidence that neurotransmitters act as morphogenic growth regulators in the developing central nervous system.Proc Natl Acad Sci U S A. 1988 Apr;85(8):2839-43. doi: 10.1073/pnas.85.8.2839. Proc Natl Acad Sci U S A. 1988. PMID: 3357895 Free PMC article.
-
Regionally Distinct Alterations in Membrane Phospholipid Metabolism in Schizophrenia: A Meta-analysis of Phosphorus Magnetic Resonance Spectroscopy Studies.Biol Psychiatry Cogn Neurosci Neuroimaging. 2020 Mar;5(3):264-280. doi: 10.1016/j.bpsc.2019.09.008. Epub 2019 Oct 1. Biol Psychiatry Cogn Neurosci Neuroimaging. 2020. PMID: 31748123 Free PMC article.
-
Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures.J Cell Biol. 1986 Jul;103(1):171-87. doi: 10.1083/jcb.103.1.171. J Cell Biol. 1986. PMID: 2424918 Free PMC article.
-
Uptake and incorporation of docosahexaenoic acid (DHA) into neuronal cell body and neurite/nerve growth cone lipids: evidence of compartmental DHA metabolism in nerve growth factor-differentiated PC12 cells.Neurochem Res. 2000 May;25(5):715-23. doi: 10.1023/a:1007575406896. Neurochem Res. 2000. PMID: 10905634
-
Synthesis of sodium channels in the cell bodies of squid giant axons.Proc Natl Acad Sci U S A. 1987 Mar;84(5):1459-63. doi: 10.1073/pnas.84.5.1459. Proc Natl Acad Sci U S A. 1987. PMID: 3469679 Free PMC article.
