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. 1983 Jan 25;11(2):489-99.
doi: 10.1093/nar/11.2.489.

Effect of several inhibitors of enzymatic DNA methylation on the in vivo methylation of different classes of DNA sequences in a cultured human cell line

D M Woodcock, J K Adams, R G Allan, I A Cooper
Free PMC article

Effect of several inhibitors of enzymatic DNA methylation on the in vivo methylation of different classes of DNA sequences in a cultured human cell line

D M Woodcock et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have previously demonstrated that, while most enzymatic formation of 5-methylcytosine in the DNA of mammalian cells occurs very shortly after strand synthesis, there is also a minor fraction of methylation which occurs in some DNA sequences up to at least several hours after strand synthesis. Using a human cell line, we have examined the effects on these two classes of enzymatic DNA methylation of several compounds which have been reported to be inhibitors of methylation reactions. We have found that cycloleucine, ethionine, and 5'-deoxy-5'-methylthioadenosine (MTA) are all effective as inhibitors of enzymatic DNA methylation, but that there is no differential effect between the delayed and non-delayed methylation reactions. Tubericidin (7-deaza-adenosine) plus homocysteine inhibited delayed DNA methylation much more than non-delayed methylation (by up to 4 times). By contrast, 5-azacytidine produced a higher level of inhibition of DNA methylation at sites in the DNA in which the methylation occurred very shortly after strand synthesis. Also 5-azacytidine was by far the most potent inhibitor of DNA methylation of the compounds tested. S-Adenosyl-homocysteine and caffeine were found to have no effect on DNA methylation. These results are discussed in relation to the number and specificity of DNA methylases in these cells and to the cellular functions of those DNA sequences in which methylation is delayed for some hours after strand synthesis.

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References

    1. Biochem J. 1973 Sep;135(1):43-57 - PubMed
    1. Aust J Exp Biol Med Sci. 1975 Aug;53(4):257-63 - PubMed
    1. Hum Genet. 1976 Dec 15;34(3):247-54 - PubMed
    1. Eur J Biochem. 1977 Nov 15;81(1):53-61 - PubMed
    1. Cancer Res. 1979 Apr;39(4):1418-24 - PubMed