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. 1981 Apr;19(3-4):321-31.
doi: 10.1007/BF00504277.

Actin acetylation in Drosophila tissue culture cells

E M Berger, G Cox, L Weber, J S Kenney

Actin acetylation in Drosophila tissue culture cells

E M Berger et al. Biochem Genet. 1981 Apr.

Abstract

In a permanent cell line derived from Drosophila embryos, cytoplasmic actin is produced as an unstable precursor, which is subsequently converted to a stable form. This conversion results in a reduction in isoelectric point, with no apparent change in molecular weight. The conversion involves an enzymatic acetylation, and results in an insensitivity to aminopeptidase digestion, suggesting N-terminal blockage. Both the acetylated and unacetylated actins can participate in the assembly of F-actin, but with different efficiencies.

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