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. 1981 Jan;31(1):214-22.
doi: 10.1128/iai.31.1.214-222.1981.

Immunochemical characterization of Brucella lipopolysaccharides and polysaccharides

E Moreno, S L Speth, L M Jones, D T Berman

Immunochemical characterization of Brucella lipopolysaccharides and polysaccharides

E Moreno et al. Infect Immun. 1981 Jan.

Abstract

Purified lipopolysaccharide (LPS) extracted with phenol-water from smooth Brucella abortus was hydrolyzed with 1% acetic acid at 100 degrees C. The degraded polysaccharide (AH) released gave reactions of identity with the native polysaccharide hapten (NH) in phenol-water- or trichloroacetic acid-extracted endotoxin preparations of B. abortus and with the polysaccharide (poly B) extracted by trichloroacetic acid from rough B. melitensis strain B115. Poly B was present in the soluble cytoplasmic fraction but not in the membrane fraction, of disrupted B115 cells. It could not be extracted from three rough mutants of B. abortus or from B canis or B. ovis cells. Both AH and NH shared determinants present on smooth LPS and missing from poly B. Sugars found in purified LPS, NH, and AH included mannose, glucose, quinovosamine, glucosamine, and 2-keto-3-deoxyoctonate. Poly B contained only a trace amount of quinovosamine and no 2-keto-3-deoxyoctonate detectable by the thiobarbiturate assay. Sera from some rabbits immunized with pure smooth LPS and some, but not all, cows infected with field strains of B. abortus recognized the determinants missing from poly B. A subclass-specific enzyme-linked immunoassay showed that most of the antibody in sera from infected cows which binds to smooth LPS and to NH is of the immunoglobulin G1 subclass.

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References

    1. J Bacteriol. 1968 Oct;96(4):893-901 - PubMed
    1. Rev Clin Esp. 1971 May;121(4):367-72 - PubMed
    1. Immunochemistry. 1971 Sep;8(9):871-4 - PubMed
    1. Eur J Biochem. 1971 Dec 22;24(1):116-22 - PubMed
    1. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):113-9 - PubMed

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