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Comparative Study
. 1984 Dec 20;3(13):3129-33.
doi: 10.1002/j.1460-2075.1984.tb02269.x.

Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor

A G Wildeman, P Sassone-Corsi, T Grundström, M Zenke, P Chambon
Comparative Study

Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor

A G Wildeman et al. EMBO J. .

Abstract

A nuclear extract prepared from HeLa cells has been used to study in vitro the transcription of the SV40 early promoter. The deletion of the enhancer results in a strong decrease of transcription, with spermidine and MgCl2 being critical variables in the transcription reactions. Furthermore a competition assay indicates that the stimulation by the enhancer is due to a specific trans-acting factor(s) which acts on it. This factor appears not to interact with SV40 or adenovirus-2 major late upstream (distal) promoter sequences, and its ability to bind to the enhancer is diminished by mutations known to decrease enhancer function in vivo and in vitro.

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