Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Sep;4(9):1915-28.
doi: 10.1128/mcb.4.9.1915-1928.1984.

Unusual regulation of simian virus 40 early-region transcription in genomes containing two origins of DNA replication

A R Buchman, P Berg

Unusual regulation of simian virus 40 early-region transcription in genomes containing two origins of DNA replication

A R Buchman et al. Mol Cell Biol. 1984 Sep.

Abstract

As part of our efforts to create multifunctional vectors for the transduction of animal cells, a set of simian virus 40 recombinants were constructed which contain an inverted duplication of the region including the origin of viral DNA replication (ori) and the early-region promoter. The unusual aspects of the structure of these recombinant genomes revealed several unexpected features of their function. In particular, transcription from the early-region promoters on these recombinants occurred primarily after the start of DNA replication, and, in that sense, these promoters behaved as if they were late-region promoters. This behavior results from the fact that these genomes contain multiple ori segments, and, therefore, they replicate earlier and faster than wild-type virus DNA, thereby causing a precocious shift in the initiation of early-region transcription from sites downstream of ori to sites located upstream of ori. The abnormal expression from multiple ori genomes is consistent with our present notions regarding the replication-dependent shift in early-region transcriptional start sites (Buchman et al., Mol. Cell. Biol. 4:1900-1914). Since our experiments demonstrate that RNAs initiated upstream of ori contribute to T-antigen formation late in infection, we suggest that the shift in early-region transcription starts modulates large T-antigen production in concert with viral DNA replication.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Appl Genet. 1982;1(4):327-41 - PubMed
    1. Nucleic Acids Res. 1984 Jan 25;12(2):915-32 - PubMed
    1. Mol Cell Biol. 1984 Sep;4(9):1900-14 - PubMed
    1. Cell. 1981 Dec;27(3 Pt 2):603-13 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

Publication types

MeSH terms